PUBLICATION
Ptehoosines a and B: Two new sesamin-type sesquilignans with antiangiogenic activity from Pterocephalus hookeri (C.B. Clarke) Höeck
- Authors
- Dong, Z.Y., Wei, L., Lu, H.Q., Zeng, Q.H., Meng, F.C., Wang, G.W., Lan, X.Z., Liao, Z.H., Chen, M.
- ID
- ZDB-PUB-210325-3
- Date
- 2021
- Source
- Fitoterapia 151: 104886 (Journal)
- Registered Authors
- Keywords
- Antiangiogenic activity, Ptehoosines a and B, Pterocephalus hookeri, Sesamin-type sesquilignan, Zebrafish model
- MeSH Terms
-
- Angiogenesis Inhibitors/isolation & purification
- Angiogenesis Inhibitors/pharmacology*
- Animals
- Caprifoliaceae/chemistry*
- Cell Cycle Checkpoints
- Dioxoles/isolation & purification
- Dioxoles/pharmacology*
- Human Umbilical Vein Endothelial Cells/drug effects
- Humans
- Lignans/isolation & purification
- Lignans/pharmacology*
- Medicine, Tibetan Traditional
- Molecular Structure
- Phytochemicals/isolation & purification
- Phytochemicals/pharmacology
- Tibet
- Zebrafish
- PubMed
- 33757847 Full text @ Fitoterapia
Citation
Dong, Z.Y., Wei, L., Lu, H.Q., Zeng, Q.H., Meng, F.C., Wang, G.W., Lan, X.Z., Liao, Z.H., Chen, M. (2021) Ptehoosines a and B: Two new sesamin-type sesquilignans with antiangiogenic activity from Pterocephalus hookeri (C.B. Clarke) Höeck. Fitoterapia. 151:104886.
Abstract
Two undescribed sesamin-type sesquilignans ptehoosines A (1) and B (2), together with 4 known lignans (3-6), were isolated from Pterocephalus hookeri (C.B. Clarke) Höeck which was widely used as traditional Tibetan medicine for treatment of rheumatoid arthritis. Their structures were determined by HR-ESI-MS, NMR analysis and CD experiment. The in vitro antiangiogenic effect of all isolated compounds against human umbilical vein endothelial cells (HUVECs) were evaluated by CCK-8 assay. Among them, compound 1 exhibited significant proliferative inhibition on HUVECs with IC50 value of 32.82 ± 0.99 μM. Further in vitro study indicated 1 could arrest cell cycle at G0/G1 phase and reduce the migration of HUVECs. In vivo experiment exhibited 1 could inhibit tail vessels plexus in zebrafish. The above finding suggested that 1 was a promising lead compound against RA by inhibiting of angiogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping