PUBLICATION
Pyroptosis Mediates Neutrophil Extracellular Trap Formation during Bacterial Infection in Zebrafish
- Authors
- Chen, W., Zhao, J., Mu, D., Wang, Z., Liu, Q., Zhang, Y., Yang, D.
- ID
- ZDB-PUB-210314-2
- Date
- 2021
- Source
- Journal of immunology (Baltimore, Md. : 1950) 206(8): 1913-1922 (Journal)
- Registered Authors
- Yang, Dahai
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Caspases/genetics
- Cells, Cultured
- Edwardsiella/physiology*
- Enterobacteriaceae Infections/immunology*
- Extracellular Traps/immunology*
- Fish Diseases/immunology*
- Hemolysin Proteins/genetics
- Immunity, Innate
- Larva
- Neutrophils/immunology*
- Pyroptosis
- Receptors, Estrogen/genetics
- Zebrafish/immunology*
- Zebrafish Proteins/genetics
- PubMed
- 33712519 Full text @ J. Immunol.
Citation
Chen, W., Zhao, J., Mu, D., Wang, Z., Liu, Q., Zhang, Y., Yang, D. (2021) Pyroptosis Mediates Neutrophil Extracellular Trap Formation during Bacterial Infection in Zebrafish. Journal of immunology (Baltimore, Md. : 1950). 206(8):1913-1922.
Abstract
The formation of neutrophil extracellular trap (NET) is a critical host defense when neutrophils migrate to infection sites. Pyroptosis is a newly identified programmed cell death, which is tightly regulated by inflammasome activation. However, the mechanism of pyroptotic signaling participating in NET production remains to be elucidated. In this study, the zebrafish larvae otic vesicle microinjection model was used to infect larvae with hemolysin-overexpressing Edwardsiella piscicida (EthA+), and a rapid migration of neutrophils to infection sites was observed. Intriguingly, EthA+ infection effectively induced significant neutrophil membrane rupture in vivo, which was dependent on caspase-B (caspy2) and gasdermin Eb (GSDMEb) but not caspase-A or gasdermin Ea. Specifically, the EthA+E. piscicida infection induced pyroptosis along with NETosis in vitro, and depletion of either caspy2 or GSDMEb impaired NET formation in vivo. Consequently, inhibition of the caspy2-GSDMEb axis-gated NETosis impaired bacterial clearance in vivo. Altogether, these data provide evidence that teleost fish innate immune cells, including neutrophils, express features of pyroptosis that are critical for NETosis in teleost innate immunity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping