PUBLICATION
Fucoxanthin-rich fraction from Sargassum fusiformis alleviates particulate matter-induced inflammation in vitro and in vivo
- Authors
- Dai, Y.L., Jiang, Y.F., Lu, Y.A., Yu, J.B., Kang, M.C., Jeon, Y.J.
- ID
- ZDB-PUB-210306-1
- Date
- 2021
- Source
- Toxicology reports 8: 349-358 (Journal)
- Registered Authors
- Jeon, You-Jin
- Keywords
- Anti-inflammatory response, COX, Cyclooxygenase, DCFH-DA, 2, 7-dichlorofluorescein diacetate, DMEM, Dulbecco's Modified Eagle's Medium, Fucoxanthin, Fx, Fucoxanthin, FxRF, Fucoxanthin-rich fraction, H-PM, Culture medium of PM-induced keratinocytes, IL, Interleukin, MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, NO, Nitric oxide, PGE, Prostaglandin E, PI, Propidium iodide, PM, Particulate matter, Particulate matter, SE, Standard error, Sargassum fusiformis, TNF-α, Tumor necrosis factor-α, iNOS, Inducible nitric oxide synthases
- MeSH Terms
- none
- PubMed
- 33665132 Full text @ Toxicol Rep
Citation
Dai, Y.L., Jiang, Y.F., Lu, Y.A., Yu, J.B., Kang, M.C., Jeon, Y.J. (2021) Fucoxanthin-rich fraction from Sargassum fusiformis alleviates particulate matter-induced inflammation in vitro and in vivo. Toxicology reports. 8:349-358.
Abstract
Particulate matter (PM) contributes to air pollution and primarily originates from unregulated industrial emissions and seasonal natural dust emissions. Fucoxanthin (Fx) is a marine natural pigment from brown macroalgae that has been shown to have various beneficial effects on health. However, the effects of Fx on PM-induced toxicities in cells and animals have not been assessed. In this study, we investigated the anti-inflammatory potential of the Fx-rich fraction (FxRF) of Sargassum fusiformis against PM-mediated inflammatory responses. The FxRF composition was analyzed by rapid-resolution liquid chromatography mass spectrometry. Fx and other main pigments were identified. FxRF attenuated the production of inflammatory components, including prostaglandin E2 (PGE2), cyclooxygenase-2, interleukin (IL)-1β, and IL-6 from PM-exposed HaCaT keratinocytes. PM exposure also reduced the levels of nitric oxide (NO), tumor necrosis factor-α, inducible nitric oxide synthase (iNOS), and PGE2 in PM-exposed RAW264.7 macrophages. Additionally, the culture medium from PM-exposed HaCaT cells induced upregulation of NO, iNOS, PGE2, and pro-inflammatory cytokines in RAW264.7 macrophages. FxRF also significantly decreased the expression levels of factors involved in inflammatory responses, such as NO, reactive oxygen species, and cell death, in PM-exposed zebrafish embryos. These results demonstrated the potential protective effects of FxRF against PM-induced inflammation both in vitro and in a zebrafish model.
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