PUBLICATION
Integrating omics and traditional analyses to profile the synergistic toxicity of graphene oxide and triphenyl phosphate
- Authors
- Zhang, X., Zhou, Q., Li, X., Zou, W., Hu, X.
- ID
- ZDB-PUB-210224-1
- Date
- 2020
- Source
- Environmental pollution (Barking, Essex : 1987) 263: 114473 (Journal)
- Registered Authors
- Keywords
- Graphene oxide, Integrated omics, Synergistic effects, Triphenyl phosphate
- MeSH Terms
-
- Animals
- Ecosystem*
- Graphite*/toxicity
- Humans
- Organophosphates/toxicity
- Zebrafish
- PubMed
- 33618456 Full text @ Environ. Pollut.
Citation
Zhang, X., Zhou, Q., Li, X., Zou, W., Hu, X. (2020) Integrating omics and traditional analyses to profile the synergistic toxicity of graphene oxide and triphenyl phosphate. Environmental pollution (Barking, Essex : 1987). 263:114473.
Abstract
The increasing production and applications of graphene oxide (GO, a novel carbon nanomaterial) have raised numerous environmental concerns regarding its ecological risks. Triphenyl phosphate (TPhP) disperses in water and poses an increasing hazard to the ecosystem and human health. It is critical to study the environmental responses and molecular mechanisms of GO and TPhP together to assess both chemicals; however, this information is lacking. The present work revealed that GO promoted the bioaccumulation of TPhP in zebrafish larvae by 5.0%-24.3%. The TPhP-induced growth inhibition of embryos (malformation, mortality, heartbeat, and spontaneous movement) at environmentally relevant concentrations was significantly amplified by GO, and these results were supported by the downregulated levels of genes and proteins associated with cytoskeletal construction and cartilage and eye development. TPhP induced negligible alterations in the genes or proteins involved in oxidative stress and apoptosis, but those related proteins were all upregulated by GO. GO and TPhP coexposure activated the mTOR signaling pathway and subsequently promoted apoptosis in zebrafish by potentiating the oxidative stress induced by TPhP, presenting synergistic toxicity. These findings highlight the potential risks and specific molecular mechanisms of combining emerging carbon nanomaterials with coexisting organic contaminants.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping