PUBLICATION
Carbamazepine induces hepatotoxicity in zebrafish by inhibition of the Wnt/β-catenin signaling pathway
- Authors
- Bai, Z., Jia, K., Chen, G., Liao, X., Cao, Z., Zhao, Y., Zhang, C., Lu, H.
- ID
- ZDB-PUB-210223-5
- Date
- 2021
- Source
- Environmental pollution (Barking, Essex : 1987) 276: 116688 (Journal)
- Registered Authors
- Keywords
- Carbamazepine, Cell apoptosis, Hepatotoxicity, Lipid accumulation, Wnt/β-catenin signal pathway, Zebrafish
- MeSH Terms
-
- Animals
- Carbamazepine/toxicity
- Chemical and Drug Induced Liver Injury*
- Larva
- Wnt Signaling Pathway
- Zebrafish*
- PubMed
- 33611196 Full text @ Environ. Pollut.
Citation
Bai, Z., Jia, K., Chen, G., Liao, X., Cao, Z., Zhao, Y., Zhang, C., Lu, H. (2021) Carbamazepine induces hepatotoxicity in zebrafish by inhibition of the Wnt/β-catenin signaling pathway. Environmental pollution (Barking, Essex : 1987). 276:116688.
Abstract
As drug abuse has become increasingly serious, carbamazepine (CBZ) is discharged into the aquatic environment with municipal sewage, causing potential harm to aquatic organisms. Here, we utilized zebrafish, an aquatic vertebrate model, to comprehensively evaluate the hepatotoxicity of CBZ. The larvae were exposed to 0.07, 0.13, and 0.26 mmol/L CBZ from 72 hpf to 144 hpf, and the adults were exposed to 0.025, 0.05, and 0.1 mmol/L CBZ for 28 days. The substantial changes were observed in the size and histopathology of livers, indicating that CBZ induced severe hepatoxicity in the larvae and adults. Oil red O staining demonstrated CBZ exposure caused severe lipid accumulation in the livers of both larvae and adults. Furthermore, CBZ exposure facilitated hepatocyte apoptosis through TUNEL staining, which was caused by rising ROS content. Subsequently, down-regulation of genes related to the Wnt pathway in exposure groups indicated that CBZ inhibited the development of liver via the Wnt/β-catenin signaling pathway. In conclusion, CBZ induced severe hepatotoxicity by promoting lipid accumulation, generating excessive ROS production, and inhibiting the Wnt/β-catenin signaling pathway in zebrafish. The results reveal the occurrence of CBZ-induced hepatotoxicity in zebrafish and clarify its mechanism of action, which potentially illustrate environmental concerns associated with CBZ exposure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping