PUBLICATION

Experimental Methodologies to Visualize Early Cell Biology in Zebrafish Embryos

Authors
Borbora, A.S., Nair, S.
ID
ZDB-PUB-210220-37
Date
2021
Source
Methods in molecular biology (Clifton, N.J.)   2218: 195-208 (Chapter)
Registered Authors
Nair, Sreelaja
Keywords
Antibody staining, Early development, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Zebrafish
MeSH Terms
  • Animals
  • Embryo, Nonmammalian/metabolism*
  • Embryo, Nonmammalian/physiology*
  • Female
  • Immunohistochemistry/methods*
  • Male
  • Zebrafish/metabolism*
  • Zebrafish/physiology*
  • Zebrafish Proteins/metabolism
PubMed
33606233 Full text @ Meth. Mol. Biol.
Abstract
For organisms to function normally, biological molecules must work at the correct time in the right cells and within the right intracellular compartments of cells. Biological research relies heavily on discovering the cellular locations at which such molecular interactions occur. A mainstay technique in this process of discovery is the visualization of locations of proteins in cells and tissues, known as immunocytochemistry and immunohistochemistry, respectively. If performed correctly, these techniques can provide detailed information regarding the endogenous locations of proteins and their ectopic locations or absence in mutants and in disease states.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping