ZFIN ID: ZDB-PUB-210220-30
Cryopreservation of Pooled Sperm Samples
Matthews, J.L., Varga, Z.M.
Date: 2021
Source: Methods in molecular biology (Clifton, N.J.)   2218: 99-115 (Chapter)
Registered Authors: Matthews, Jennifer, Varga, Zoltan M.
Keywords: Cell density, Cell motility, Cryopreservation, In vitro fertilization, Protocol, Quality assessment, Quality control, Zebrafish (Danio rerio)
MeSH Terms:
  • Animals
  • Cryopreservation/methods*
  • Cryoprotective Agents/pharmacology
  • Fertilization in Vitro/methods
  • Male
  • Semen Preservation/methods*
  • Sperm Motility/drug effects
  • Sperm Motility/physiology
  • Spermatozoa/cytology*
  • Spermatozoa/drug effects
  • Zebrafish/physiology
PubMed: 33606226 Full text @ Meth. Mol. Biol.
Cryopreservation of sperm cells is currently the most efficient tool for managing large and small collections of valuable genetic resources. Cryopreservation minimizes expenses for animal and facility maintenance such as personnel, water, power, and space. It extends the time offspring can be produced from individual organisms, reduces the need to maintain live populations, provides flexibility for planning future experiments and research projects, and can prevent catastrophic loss of irreplaceable research lines. In this chapter, we present the sperm collection, dilution, cryopreservation, thawing, and in vitro fertilization procedures used at the Zebrafish International Resource Center (ZIRC).