ZFIN ID: ZDB-PUB-210220-29
Applying Rho Pathway Inhibitors to Investigate Germ Plasm Localization
Miranda, J., Schnabel, D.
Date: 2021
Source: Methods in molecular biology (Clifton, N.J.)   2218: 85-97 (Chapter)
Registered Authors: Schnabel, Denhi
Keywords: Cytoskeleton, Germ line, ROCK, Rho GTPases, RhoA, Zebrafish, mRNA localization
MeSH Terms:
  • Animals
  • Cytoplasm/metabolism
  • Cytoskeleton/metabolism
  • Embryo, Nonmammalian/metabolism
  • Female
  • Germ Cells/metabolism*
  • Germ Cells/physiology*
  • Male
  • Microtubules/metabolism
  • RNA, Messenger/metabolism
  • Signal Transduction/physiology*
  • Zebrafish/metabolism
  • Zebrafish Proteins/metabolism
  • rhoA GTP-Binding Protein/metabolism*
PubMed: 33606225 Full text @ Meth. Mol. Biol.
ABSTRACT
The correct assembly, migration, and segregation of the mRNAs of the germ plasm during the first cell divisions are intimately connected to the cytoskeleton and cytokinesis.RhoA is a key regulator of germ plasm localization during the first two cell division cycles in zebrafish embryos. Pharmacological inhibition of RhoA and his effector ROCK affected the correct assembly of microtubules in the cleavage furrow with the concomitant abnormal localization of germ plasm mRNAs. The inhibition of RhoA/ROCK pathway caused a significant decrease in the germ cell population later in development.
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