PUBLICATION
Anxiolytic, Promnesic, Anti-Acetylcholinesterase and Antioxidant Effects of Cotinine and 6-Hydroxy-L-Nicotine in Scopolamine-Induced Zebrafish (Danio rerio) Model of Alzheimer's Disease
- Authors
- Boiangiu, R.S., Mihasan, M., Gorgan, D.L., Stache, B.A., Hritcu, L.
- ID
- ZDB-PUB-210205-2
- Date
- 2021
- Source
- Antioxidants (Basel, Switzerland) 10(2): (Journal)
- Registered Authors
- Keywords
- 6-hydroxy-L-nicotine, Alzheimer’s disease, acetylcholinesterase, anxiety, cotinine, memory, nicotine, oxidative stress, scopolamine, zebrafish
- MeSH Terms
- none
- PubMed
- 33535660 Full text @ Antioxidants (Basel)
Citation
Boiangiu, R.S., Mihasan, M., Gorgan, D.L., Stache, B.A., Hritcu, L. (2021) Anxiolytic, Promnesic, Anti-Acetylcholinesterase and Antioxidant Effects of Cotinine and 6-Hydroxy-L-Nicotine in Scopolamine-Induced Zebrafish (Danio rerio) Model of Alzheimer's Disease. Antioxidants (Basel, Switzerland). 10(2):.
Abstract
Cotinine (COT) and 6-hydroxy-L-nicotine (6HLN) are two nicotinic derivatives that possess cognitive-improving abilities and antioxidant properties in different rodent models of Alzheimer's disease (AD), eluding the side-effects of nicotine (NIC), the parent molecule. In the current study, we evaluated the impact of COT and 6HLN on memory deterioration, anxiety, and oxidative stress in the scopolamine (SCOP)-induced zebrafish model of AD. For this, COT and 6HLN were acutely administered by immersion to zebrafish that were treated with SCOP before testing. The memory performances were assessed in Y-maze and object discrimination (NOR) tasks, while the anxiety-like behavior was evaluated in the novel tank diving test (NTT). The acetylcholinesterase (AChE) activity and oxidative stress were measured from brain samples. The RT-qPCR analysis was used to evaluate the npy, egr1, bdnf, and nrf2a gene expression. Our data indicated that both COT and 6HLN attenuated the SCOP-induced anxiety-like behavior and memory impairment and reduced the oxidative stress and AChE activity in the brain of zebrafish. Finally, RT-qPCR analysis indicated that COT and 6HLN increased the npy, egr1, bdnf, and nrf2a gene expression. Therefore, COT and 6HLN could be used as tools for improving AD conditions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping