PUBLICATION
Benfuresate induces developmental toxicity in zebrafish larvae by generating apoptosis and pathological modifications
- Authors
- Lee, J.Y., Park, H., Lim, W., Song, G.
- ID
- ZDB-PUB-210202-4
- Date
- 2021
- Source
- Pesticide biochemistry and physiology 172: 104751 (Journal)
- Registered Authors
- Keywords
- Abnormal vascular development, Apoptosis, Benfuresate, Malformation, Zebrafish larvae
- MeSH Terms
-
- Animals
- Apoptosis
- Benzofurans
- Embryo, Nonmammalian
- Larva
- Sulfonic Acids
- Water Pollutants, Chemical*
- Zebrafish*/genetics
- PubMed
- 33518044 Full text @ Pestic Biochem Physiol
Citation
Lee, J.Y., Park, H., Lim, W., Song, G. (2021) Benfuresate induces developmental toxicity in zebrafish larvae by generating apoptosis and pathological modifications. Pesticide biochemistry and physiology. 172:104751.
Abstract
Benfuresate (2,3-dihydro-3,3-dimethylbenzofuran-5-yl ethanesulphonate) is a widely used pre-emergence herbicide of the benzofurane group, which works through the inhibition of lipid synthesis. During embryonic development of zebrafish, benfuresate retards growth while causing internal changes in the body, including alteration of the expression of cell cycle regulators, induction of apoptosis, and suppression of the circulatory system. Acute toxicity towards benfuresate is seen across the range of 5-15 μM in a dose-dependent manner and contributes to pathological conditions and subsequent morphological changes. For embryos 120 h post fertilization (hpf), benfuresate exposure results in an array of malformations involving eye or otolith development, pericardial edema, yolk sac edema, and abnormal curvature of the spine. Mechanistically, benfuresate exposure altered the transcription levels of the proliferative pathway genes ccnd1, ccne1, cdk2, and cdk6, all of which sensitize cells to apoptosis. Benfuresate exposure also affected vascular formation, including the formation of various vessels (DA, SIVs, CA, CV) whose functions in lymphatic-blood circulation were disrupted following decreased vegfaa, vegfc, flt1, flt4, and kdrl expression. These findings provide evidence of embryo-larval toxicity due to benfuresate and highlight the perils of herbicide exposure for non-target organisms far removed from application sites, especially in aquatic environments.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping