PUBLICATION

Functional Role of the RNA-Binding Protein Rbm24a and Its Target sox2 in Microphthalmia

Authors
Brastrom, L.K., Scott, C.A., Wang, K., Slusarski, D.C.
ID
ZDB-PUB-210128-1
Date
2021
Source
Biomedicines   9(2): (Journal)
Registered Authors
Slusarski, Diane C.
Keywords
RNA binding protein, microphthalmia, post-transcriptional regulation, rbm24a, sox2, vision, visual assay, zebrafish
MeSH Terms
none
PubMed
33494192 Full text @ Biomedicines
Abstract
Congenital eye defects represent a large class of disorders affecting roughly 21 million children worldwide. Microphthalmia and anophthalmia are relatively common congenital defects, with approximately 20% of human cases caused by mutations in SOX2. Recently, we identified the RNA-binding motif protein 24a (Rbm24a) which binds to and regulates sox2 in zebrafish and mice. Here we show that morpholino knockdown of rbm24a leads to microphthalmia and visual impairment. By utilizing sequential injections, we demonstrate that addition of exogenous sox2 RNA to rbm24a-deplete embryos is sufficient to suppress morphological and visual defects. This research demonstrates a critical role for understanding the post-transcriptional regulation of genes needed for development.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
No data available
Phenotype
Fish Conditions Stage Phenotype Figure
WT + CRISPR4-rbm24astandard conditionsDay 4
WT + CRISPR4-rbm24astandard conditionsDay 4
WT + MO6-rbm24astandard conditionsDay 4
WT + MO6-rbm24astandard conditionsDay 4
WT + MO6-rbm24astandard conditionsDay 4
WT + MO6-rbm24astandard conditionsDay 4
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Mutations / Transgenics
No data available
Human Disease / Model
Human Disease Fish Conditions Evidence
microphthalmiaTAS
1 - 1 of 1
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Sequence Targeting Reagents
Target Reagent Reagent Type
rbm24aCRISPR4-rbm24aCRISPR
rbm24aMO6-rbm24aMRPHLNO
rbm24aMO7-rbm24aMRPHLNO
1 - 3 of 3
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Fish
Fish
WT + CRISPR4-rbm24a
WT + MO6-rbm24a
1 - 2 of 2
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Antibodies
No data available
Orthology
Gene Orthology
rbm24a
1 - 1 of 1
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Engineered Foreign Genes
No data available
Mapping
No data available
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Citation
Brastrom, L.K., Scott, C.A., Wang, K., Slusarski, D.C. (2021) Functional Role of the RNA-Binding Protein Rbm24a and Its Target sox2 in Microphthalmia. Biomedicines. 9(2):.