PUBLICATION
Late onset of Synaptotagmin 2a expression at synapses relevant to social behavior
- Authors
- Goode, C., Voeun, M., Ncube, D., Eisen, J., Washbourne, P., Tallafuss, A.
- ID
- ZDB-PUB-210126-13
- Date
- 2020
- Source
- The Journal of comparative neurology 529(9): 2176-2188 (Journal)
- Registered Authors
- Eisen, Judith S., Tallafuss, Alexandra, Washbourne, Philip
- Keywords
- Synaptotagmin, social behavior, synapse, tyrosine hydroxylase, ventral forebrain, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Gene Expression
- Prosencephalon/embryology
- Prosencephalon/metabolism*
- Social Behavior*
- Synapses/genetics
- Synapses/metabolism*
- Synaptotagmin II/biosynthesis*
- Synaptotagmin II/genetics
- Zebrafish
- PubMed
- 33491202 Full text @ J. Comp. Neurol.
Citation
Goode, C., Voeun, M., Ncube, D., Eisen, J., Washbourne, P., Tallafuss, A. (2020) Late onset of Synaptotagmin 2a expression at synapses relevant to social behavior. The Journal of comparative neurology. 529(9):2176-2188.
Abstract
As they form, synapses go through various stages of maturation and refinement. These steps are linked to significant changes in synaptic function, potentially resulting in emergence and maturation of behavioral outputs. Synaptotagmins are calcium-sensing proteins of the synaptic vesicle exocytosis machinery, and changes in Synaptotagmin proteins at synapses have significant effects on vesicle release and synaptic function. Here, we examined the distribution of the synaptic vesicle protein Synaptotagmin 2a (Syt2a) during development of the zebrafish nervous system. Syt2a is widely distributed throughout the midbrain and hindbrain early during larval development but very weakly expressed in the forebrain. Later in development, Syt2a expression levels in the forebrain increase, particularly in regions associated with social behavior, and most intriguingly, around the time social behavior becomes apparent. We provide evidence that Syt2a localizes to synapses onto neurons implicated in social behavior in the ventral forebrain and show that Syt2a is colocalized with tyrosine hydroxylase, a biosynthetic enzyme in the dopamine pathway. Our results suggest a developmentally important role for Syt2a in maturing synapses in the forebrain, coinciding with the emergence of social behavior.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping