PUBLICATION
Structural properties and in vitro and in vivo immunomodulatory activity of an arabinofuranan from the fruits of Akebia quinata
- Authors
- Wang, H., Wang, X., Li, Y., Zhang, S., Li, Z., Li, Y., Cui, J., Lan, X., Zhang, E., Yuan, L., Jin, D.Q., Tuerhong, M., Abudukeremu, M., Xu, J., Guo, Y.
- ID
- ZDB-PUB-210124-1
- Date
- 2021
- Source
- Carbohydrate Polymers 256: 117521 (Journal)
- Registered Authors
- Cui, Jianlin, Li, Yuhao
- Keywords
- Akebia quinata, Arabinofuranan, Immunoregulatory activity, Methyl iodide (PubChem CID: 6328), Neutral red (PubChem CID: 11105), O-tolylisothiocyanate (PubChem CID: 341280687), Pyridine (PubChem CID: 1049), RAW264.7 macrophage cells, Trifluoroacetic acid (PubChem CID: 6422), Zebrafish, d-Arabinose (PubChem CID: 854), d-Mannose (PubChem CID: 18950), l-Arabinose (PubChem CID: 439195), l-Fucose (PubChem CID: 17106), n-Butanol (PubChem CID: 341280687)
- MeSH Terms
-
- Animals
- Polysaccharides/chemistry*
- Polysaccharides/isolation & purification
- Polysaccharides/pharmacology
- Phagocytosis/drug effects
- Immunologic Factors/chemistry*
- Immunologic Factors/isolation & purification
- Immunologic Factors/pharmacology
- Fruit/chemistry*
- Ranunculales/chemistry*
- Tumor Necrosis Factor-alpha/immunology
- Tumor Necrosis Factor-alpha/metabolism
- Nitric Oxide/immunology
- Nitric Oxide/metabolism
- Carbohydrate Sequence
- Stereoisomerism
- Molecular Weight
- Zebrafish
- Interleukin-6/immunology
- Interleukin-6/metabolism
- Reactive Oxygen Species/agonists*
- Reactive Oxygen Species/immunology
- Reactive Oxygen Species/metabolism
- Interleukin-1beta/immunology
- Interleukin-1beta/metabolism
- Mice
- RAW 264.7 Cells
- Embryo, Nonmammalian
- Plant Extracts/chemistry
- PubMed
- 33483042 Full text @ Carbohydr. Polym.
Citation
Wang, H., Wang, X., Li, Y., Zhang, S., Li, Z., Li, Y., Cui, J., Lan, X., Zhang, E., Yuan, L., Jin, D.Q., Tuerhong, M., Abudukeremu, M., Xu, J., Guo, Y. (2021) Structural properties and in vitro and in vivo immunomodulatory activity of an arabinofuranan from the fruits of Akebia quinata. Carbohydrate Polymers. 256:117521.
Abstract
In our continuous searching for natural active polysaccharides with immunomodulatory activity, an arabinofuranan (AQP70-3) was isolated and purified from the fruits of Akebia quinata (Houtt.) Decne. by using ion-exchange chromatography and gel permeation chromatography for the first time. AQP70-3 contained both α-l-Araf and β-l-Araf, and the absolute molecular weight was 1.06 × 104 g/mol. The backbone of AQP70-3 comprised →5)-α-l-Araf-(1→, →3,5)-α-l-Araf-(1→, and →2,5)-α-l-Araf-(1→, with branches of →1)-β-l-Arafand →3)-α-l-Araf-(1→ residues. Biological assay suggested that AQP70-3 can stimulate phagocytic activity and promote the levels of nitric oxide (NO), interleukin (IL)-6, IL-1β, and tumor necrosis factor-α (TNF-α) of RAW264.7 cells. Furthermore, AQP70-3 was found to increase the production of reactive oxygen species (ROS) and NO in zebrafish embryo model.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping