PUBLICATION

Anterior lateral plate mesoderm gives rise to multiple tissues and requires tbx5a function in left-right asymmetry, migration dynamics, and cell specification of late-addition cardiac cells

Authors
Mao, L.M.F., Boyle Anderson, E.A.T., Ho, R.K.
ID
ZDB-PUB-210123-29
Date
2021
Source
Developmental Biology   472: 52-66 (Journal)
Registered Authors
Ho, Robert K.
Keywords
Cardiac development, Fate map, Lateral plate mesoderm, Left-right asymmetry, Zebrafish, tbx5a
MeSH Terms
  • Transcription Factors/deficiency*
  • Transcription Factors/genetics*
  • Signal Transduction/genetics
  • Myocytes, Cardiac/metabolism*
  • Embryo, Nonmammalian/metabolism
  • Branchial Region/metabolism
  • Cell Movement/genetics*
  • Gene Knockdown Techniques
  • Animals, Genetically Modified
  • Cell Differentiation/genetics*
  • Body Patterning/genetics*
  • Gene Expression Regulation, Developmental
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism*
  • Zebrafish Proteins/deficiency*
  • Zebrafish Proteins/genetics*
  • Animals
  • Mesoderm/metabolism*
  • Organogenesis/genetics
(all 20)
PubMed
33482174 Full text @ Dev. Biol.
Abstract
In this study, we elucidate a single cell resolution fate map in the zebrafish in a sub-section of the anterior Lateral Plate Mesoderm (aLPM) at 18 hpf. Our results show that this tissue is not organized into segregated regions but gives rise to intermingled pericardial sac, peritoneum, pharyngeal arch and cardiac precursors. We further report upon asymmetrical contributions of lateral aLPM-derived heart precursors-specifically that twice as many heart precursors arise from the right side versus the left side of the embryo. Cell tracking analyses and large-scale cell labeling of the lateral aLPM corroborate these differences and show that the observed asymmetries are dependent upon Tbx5a expression. Previously, it was shown that cardiac looping was affected in Tbx5a knock-down and knock-out zebrafish (Garrity et al., 2002; Parrie et al., 2013); our present data also implicate tbx5a function in cell specification, establishment and maintenance of cardiac left-right asymmetry.
Genes / Markers
Figures
Figure Gallery (6 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ch2TgTransgenic Insertion
mw3TgTransgenic Insertion
    1 - 2 of 2
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    tbx5aMO2-tbx5aMRPHLNO
    1 - 1 of 1
    Show
    Fish
    No data available
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    EGFPEFGEGFP
    mCherryEFGmCherry
    1 - 2 of 2
    Show
    Mapping
    No data available
    Your Input Welcome
    We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
    Citation
    Mao, L.M.F., Boyle Anderson, E.A.T., Ho, R.K. (2021) Anterior lateral plate mesoderm gives rise to multiple tissues and requires tbx5a function in left-right asymmetry, migration dynamics, and cell specification of late-addition cardiac cells. Developmental Biology. 472:52-66.