ZFIN ID: ZDB-PUB-210123-26
Cellular and Molecular Characterization of the Effects of the Zebrafish Embryo Genotyper Protocol
Douek, A.M., Klein, E.I., Kaslin, J., Currie, P.D., Ruparelia, A.A.
Date: 2021
Source: Zebrafish   18(1): 92-95 (Journal)
Registered Authors: Currie, Peter D., Kaslin, Jan, Ruparelia, Avnika
Keywords: ZEG, zebrafish embryo genotyper, zebrafish genotyping
MeSH Terms: none
PubMed: 33481695 Full text @ Zebrafish
The Zebrafish Embryo Genotyper (ZEG) device provides a promising tool for genotyping live embryos. Although the gross morphology and survival of embryos after the use of ZEG are unaffected, the cellular and molecular effects of the ZEG protocol remain unknown. To address this, we have examined the integrity of specific tissues, and evaluated the expression of stress-responsive genes to determine the impact of the ZEG protocol. Our analyses reveal that although ZEG results in a low-level acute stress response, no long-lasting effects are evident, supporting its utilization for a variety of downstream assays.