|ZFIN ID: ZDB-PUB-210123-26|
Cellular and Molecular Characterization of the Effects of the Zebrafish Embryo Genotyper Protocol
Douek, A.M., Klein, E.I., Kaslin, J., Currie, P.D., Ruparelia, A.A.
|Source:||Zebrafish 18(1): 92-95 (Journal)|
|Registered Authors:||Currie, Peter D., Kaslin, Jan, Ruparelia, Avnika|
|Keywords:||ZEG, zebrafish embryo genotyper, zebrafish genotyping|
|PubMed:||33481695 Full text @ Zebrafish|
Douek, A.M., Klein, E.I., Kaslin, J., Currie, P.D., Ruparelia, A.A. (2021) Cellular and Molecular Characterization of the Effects of the Zebrafish Embryo Genotyper Protocol. Zebrafish. 18(1):92-95.
ABSTRACTThe Zebrafish Embryo Genotyper (ZEG) device provides a promising tool for genotyping live embryos. Although the gross morphology and survival of embryos after the use of ZEG are unaffected, the cellular and molecular effects of the ZEG protocol remain unknown. To address this, we have examined the integrity of specific tissues, and evaluated the expression of stress-responsive genes to determine the impact of the ZEG protocol. Our analyses reveal that although ZEG results in a low-level acute stress response, no long-lasting effects are evident, supporting its utilization for a variety of downstream assays.
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