PUBLICATION
Insulin-Like Growth Factor-1 Enhances Motoneuron Survival and Inhibits Neuroinflammation After Spinal Cord Transection in Zebrafish
- Authors
- Zhao, L., Zhang, B., Huang, S., Zhou, Z., Jia, X., Qiao, C., Wang, F., Sun, M., Shi, Y., Yao, L., Cui, C., Shen, Y.
- ID
- ZDB-PUB-210123-24
- Date
- 2021
- Source
- Cellular and molecular neurobiology 42(5): 1373-1384 (Journal)
- Registered Authors
- Keywords
- Insulin-like growth factor-1, Motoneuron, Neuroinflammation, Spinal cord transection, Zebrafish
- MeSH Terms
-
- Nerve Regeneration/physiology
- Neuroinflammatory Diseases
- Animals
- Spinal Cord/metabolism
- Insulin-Like Growth Factor I/metabolism
- Insulin-Like Growth Factor I/pharmacology
- Zebrafish*
- Axons/metabolism
- Spinal Cord Injuries*/metabolism
- Mammals
- Motor Neurons/metabolism
- PubMed
- 33481118 Full text @ Cell. Mol. Neurobiol.
Citation
Zhao, L., Zhang, B., Huang, S., Zhou, Z., Jia, X., Qiao, C., Wang, F., Sun, M., Shi, Y., Yao, L., Cui, C., Shen, Y. (2021) Insulin-Like Growth Factor-1 Enhances Motoneuron Survival and Inhibits Neuroinflammation After Spinal Cord Transection in Zebrafish. Cellular and molecular neurobiology. 42(5):1373-1384.
Abstract
Insulin-like growth factor-1 (IGF-1) is a neurotrophic factor produced locally in the central nervous system which can promote axonal regeneration, protect motoneurons, and inhibit neuroinflammation. In this study, we used the zebrafish spinal transection model to investigate whether IGF-1 plays an important role in the recovery of motor function. Unlike mammals, zebrafish can regenerate axons and restore mobility in remarkably short period after spinal cord transection. Quantitative real-time PCR and immunofluorescence showed decreased IGF-1 expression in the lesion site. Double immunostaining for IGF-1 and Islet-1 (motoneuron marker)/GFAP (astrocyte marker)/Iba-1 (microglia marker) showed that IGF-1 was mainly expressed in motoneurons and was surrounded by astrocyte and microglia. Following administration of IGF-1 morpholino at the lesion site of spinal-transected zebrafish, swimming test showed retarded recovery of mobility, the number of motoneurons was reduced, and increased immunofluorescence density of microglia was caused. Our data suggested that IGF-1 enhances motoneuron survival and inhibits neuroinflammation after spinal cord transection in zebrafish, which suggested that IGF-1 might be involved in the motor recovery.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping