PUBLICATION
Organism dual RNA-seq reveals the importance of BarA/UvrY in Vibrio parahaemolyticus virulence
- Authors
- Zhang, W., Xie, R., Zhang, X.D., Lee, L.T.O., Zhang, H., Yang, M., Peng, B., Zheng, J.
- ID
- ZDB-PUB-210120-27
- Date
- 2020
- Source
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34: 7561-7577 (Journal)
- Registered Authors
- Keywords
- C. elegans, V. parahaemolyticus, BarA, UvrY, organism dual RNA-Seq, virulence factor
- MeSH Terms
-
- Animals
- Bacterial Proteins/genetics*
- Caenorhabditis elegans/microbiology
- Cell Line, Tumor
- HeLa Cells
- Host-Pathogen Interactions/genetics
- Humans
- Immunity, Innate/genetics
- Membrane Proteins/genetics
- RNA-Seq/methods
- Transcription Factors/genetics
- Vibrio parahaemolyticus/genetics*
- Virulence/genetics*
- Virulence Factors/genetics*
- Zebrafish
- PubMed
- 32281204 Full text @ FASEB J.
Citation
Zhang, W., Xie, R., Zhang, X.D., Lee, L.T.O., Zhang, H., Yang, M., Peng, B., Zheng, J. (2020) Organism dual RNA-seq reveals the importance of BarA/UvrY in Vibrio parahaemolyticus virulence. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 34:7561-7577.
Abstract
Elucidation of host-pathogen interaction is essential for developing effective strategies to combat bacterial infection. Dual RNA-Seq using cultured cells or tissues/organs as the host of pathogen has emerged as a novel strategy to understand the responses concurrently from both pathogen and host at cellular level. However, bacterial infection mostly causes systematic responses from the host at organism level where the interplay is urgently to be understood but inevitably being neglected by the current practice. Here, we developed an approach that simultaneously monitor the genome-wide infection-linked transcriptional alterations in both pathogenic Vibrio parahaemolyticus and the infection host nematode Caenorhabditis elegans. Besides the dynamic alterations in transcriptomes of both C. elegans and V. parahaemolyticus during infection, we identify a two-component system, BarA/UvrY, that is important for virulence in host. BarA/UvrY not only controls the virulence factors in V. parahaemolyticus including Type III and Type VI secretion systems, but also attenuates innate immune responses in C. elegans, including repression on the MAP kinase-mediated cascades. Thus, our study exemplifies the use of dual RNA-Seq at organism level to uncover previously unrecognized interplay between host and pathogen.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping