PUBLICATION
Ca2+ plays an antiviral role by increasing p53 expression to achieve protection against spring viraemia of carp virus infection
- Authors
- Lu, L.F., Zhou, X.Y., Zhang, C., Li, Z.C., Chen, D.D., Zhang, Y.A., Li, S.
- ID
- ZDB-PUB-210116-11
- Date
- 2020
- Source
- Fish & shellfish immunology 102: 449-459 (Journal)
- Registered Authors
- Keywords
- Antiviral, Ca(2+), SVCV, Ubiquitination, p53
- MeSH Terms
-
- Animals
- Antiviral Agents/pharmacology*
- Calcium/pharmacology*
- Cell Line
- Cyprinidae
- Female
- Fish Diseases/immunology*
- Fish Proteins/metabolism*
- HEK293 Cells
- Humans
- Male
- Rhabdoviridae/physiology
- Rhabdoviridae Infections/immunology
- Rhabdoviridae Infections/veterinary
- Tumor Suppressor Protein p53/metabolism*
- Zebrafish/immunology*
- PubMed
- 32408017 Full text @ Fish Shellfish Immunol.
Citation
Lu, L.F., Zhou, X.Y., Zhang, C., Li, Z.C., Chen, D.D., Zhang, Y.A., Li, S. (2020) Ca2+ plays an antiviral role by increasing p53 expression to achieve protection against spring viraemia of carp virus infection. Fish & shellfish immunology. 102:449-459.
Abstract
Calcium (Ca) is a messenger that regulates a multitude of physiological processes, but its functions in antiviral progress remain undefined. In this study, we found that Ca2+ enhances fish survival to defend against spring viraemia of carp virus (SVCV) infection by reversing the instability of p53 mediated by the viral protein. First, Ca2+ significantly protected cells and fish against SVCV infection by inducing early apoptosis. Additionally, p53 expression, which was inhibited by SVCV N protein, was upregulated by Ca2+ treatment. Then, the mechanism underlying the reduction of K63-linked p53 ubiquitination by SVCV N protein via the K358 site was completely prevented by Ca2+. These findings reveal the role of Ca2+ in lower vertebrates in the antiviral response, which is connected to and corresponds with viral immune evasion, providing a solution to fish diseases caused by pathogens.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping