PUBLICATION
NF-Y is critical for the proper growth of zebrafish embryonic heart and its cardiomyocyte proliferation
- Authors
- Chen, J.
- ID
- ZDB-PUB-210109-28
- Date
- 2021
- Source
- Genesis (New York, N.Y. : 2000) 59(1-2): e23408 (Journal)
- Registered Authors
- Keywords
- NF-Y, cardiomyocyte proliferation, development, embryonic heart, zebrafish
- MeSH Terms
-
- Animals
- CCAAT-Binding Factor/genetics*
- CCAAT-Binding Factor/metabolism
- Cell Proliferation
- Cells, Cultured
- Heart/embryology*
- Myocytes, Cardiac/metabolism*
- Myocytes, Cardiac/physiology
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 33417743 Full text @ Genesis
Citation
Chen, J. (2021) NF-Y is critical for the proper growth of zebrafish embryonic heart and its cardiomyocyte proliferation. Genesis (New York, N.Y. : 2000). 59(1-2):e23408.
Abstract
The ubiquitous NF-Y gene regulates the expression of different genes in various signaling pathways. However, the function of NF-Y in zebrafish heart development is largely unknown. Previously we identified a same group of cell cycle related gene cluster (CCRG) was downregulated in the embryonic hearts with impeded growth due to various stresses. The promoter regions of these CCRG genes shared a most common motif for NF-Y. Chromatin immunoprecipitation experiment demonstrated that the binding of NF-Y to its motif was real on the CCRG candidate gene promoters. Knockdown of embryonic NF-Y by morpholinos led to a small heart, mimicking the abnormal heart phenotype caused by other stresses. In parallel the expression of certain CCRG candidate genes was reduced in the NF-Y A morphant hearts exposed to malignant environments. Absence of NF-Y A also led to undermine cardiomyocyte proliferation and hence less total number of caridomyocytes per heart. Trans-AM Elisa experiment also found that in the presence of the stresses such as TCDD and TNNT2 MO, the binding capacity of NF-Y A subunit to its core motif was reduced. We conclude that NF-Y sustains proper cardiomyocyte proliferation in the heart, thus it plays a positive role in promoting early zebrafish heart growth.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping