PUBLICATION
Disruption of Abcc6 Transporter in Zebrafish Causes Ocular Calcification and Cardiac Fibrosis
- Authors
- Sun, J., She, P., Liu, X., Gao, B., Jin, D., Zhong, T.P.
- ID
- ZDB-PUB-210103-1
- Date
- 2020
- Source
- International Journal of Molecular Sciences 22(1): (Journal)
- Registered Authors
- Jin, Daqing, Zhong, Tao P.
- Keywords
- ABCC6, PXE, cardiac fibrosis, ocular calcification, vitamin K
- MeSH Terms
-
- ATP-Binding Cassette Transporters/genetics*
- ATP-Binding Cassette Transporters/metabolism
- Animals
- Calcinosis/genetics*
- Calcinosis/pathology*
- Eye/metabolism
- Eye/pathology
- Fibrosis/genetics*
- Fibrosis/pathology*
- Genetic Predisposition to Disease
- Mutation
- Myocardium/metabolism
- Myocardium/pathology
- Vitamin K/metabolism
- Vitamin K/pharmacology
- Zebrafish
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 33383974 Full text @ Int. J. Mol. Sci.
Citation
Sun, J., She, P., Liu, X., Gao, B., Jin, D., Zhong, T.P. (2020) Disruption of Abcc6 Transporter in Zebrafish Causes Ocular Calcification and Cardiac Fibrosis. International Journal of Molecular Sciences. 22(1):.
Abstract
Pseudoxanthoma elasticum (PXE), caused by ABCC6/MRP6 mutation, is a heritable multisystem disorder in humans. The progressive clinical manifestations of PXE are accompanied by ectopic mineralization in various connective tissues. However, the pathomechanisms underlying the PXE multisystem disorder remains obscure, and effective treatment is currently available. In this study, we generated zebrafish abcc6a mutants using the transcription activator-like effector nuclease (TALEN) technique. In young adult zebrafish, abcc6a is expressed in the eyes, heart, intestine, and other tissues. abcc6a mutants exhibit extensive calcification in the ocular sclera and Bruch's membrane, recapitulating part of the PXE manifestations. Mutations in abcc6a upregulate extracellular matrix (ECM) genes, leading to fibrotic heart with reduced cardiomyocyte number. We found that abcc6a mutation reduced levels of both vitamin K and pyrophosphate (PPi) in the serum and diverse tissues. Vitamin K administration increased the gamma-glutamyl carboxylated form of matrix gla protein (cMGP), alleviating ectopic calcification and fibrosis in vertebrae, eyes, and hearts. Our findings contribute to a comprehensive understanding of PXE pathophysiology from zebrafish models.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping