PUBLICATION
Saringosterol acetate isolated from Hizikia fusiforme, an edible brown alga, suppressed hepatocellular carcinoma growth and metastasis in a zebrafish xenograft model
- Authors
- Kim, E.A., Lee, J.H., Heo, S.J., Jeon, Y.J.
- ID
- ZDB-PUB-201229-22
- Date
- 2020
- Source
- Chemico-biological interactions 335: 109362 (Journal)
- Registered Authors
- Keywords
- Hizikia fusiforme, Saringosterol acetate, hepatocellular carcinoma, zebrafish xenograft model
- MeSH Terms
-
- Antineoplastic Agents/therapeutic use*
- Sargassum/chemistry*
- Humans
- Female
- Animals
- Carcinoma, Hepatocellular/drug therapy*
- Zebrafish
- Signal Transduction/drug effects
- Cell Line, Tumor
- Neoplasm Metastasis/drug therapy
- Male
- Xenograft Model Antitumor Assays
- Stigmasterol/analogs & derivatives*
- Stigmasterol/therapeutic use*
- Liver Neoplasms/drug therapy*
- PubMed
- 33358999 Full text @ Chem. Biol. Interact.
Citation
Kim, E.A., Lee, J.H., Heo, S.J., Jeon, Y.J. (2020) Saringosterol acetate isolated from Hizikia fusiforme, an edible brown alga, suppressed hepatocellular carcinoma growth and metastasis in a zebrafish xenograft model. Chemico-biological interactions. 335:109362.
Abstract
Saringosterol acetate (SSA) was isolated from an edible brown alga Hizikia fusiforme. In this study, we developed an adult zebrafish human hepatocellular carcinoma (HCC) xenograft model to confirm that SSA inhibits tumor growth and metastasis. Established Hep3B cells labeled with the fluorescent tracker CM-Dil were xenografted into the abdominal cavity of zebrafish once every three days for one month. Compared with the control group, the fish injected with Hep3B showed a significant increase in α-fetoprotein (AFP) and factors related to tumor growth and metastasis (IL-6, TNF-α, TGFβ, MMP2, and MMP9). Using the model, it was proven that SSA affected survival rate, AFP production, and the levels of factors related to tumor growth and metastasis via the PI3K/AKT/mTOR and TGFβ/Smad pathways. In conclusion, this HCC model can be used for in vivo experiments to investigate the inhibition of cancer, and SSA may be useful for the treatment of cancer.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping