PUBLICATION
Characterization of novel zebrafish MHC class I U lineage genes and their haplotype
- Authors
- Honjo, Y., Takano, K., Ichinohe, T.
- ID
- ZDB-PUB-201208-48
- Date
- 2020
- Source
- Developmental and comparative immunology 116: 103952 (Journal)
- Registered Authors
- Keywords
- major histocompatibility complex, zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Chromosomes/genetics
- Gene Expression
- Gene Order
- Genes, MHC Class I*
- Haplotypes
- Histocompatibility Antigens Class I/genetics*
- Phylogeny
- Tissue Distribution
- Zebrafish/genetics*
- Zebrafish/immunology
- Zebrafish Proteins/genetics
- PubMed
- 33279476 Full text @ Dev. Comp. Immunol.
Citation
Honjo, Y., Takano, K., Ichinohe, T. (2020) Characterization of novel zebrafish MHC class I U lineage genes and their haplotype. Developmental and comparative immunology. 116:103952.
Abstract
Major histocompatibility complex (MHC) genes are essential for distinguishing between individuals in all jawed vertebrates. Although MHC class I (mhc1) genes in zebrafish comprise distinct haplotypes, not all members of the mhc1 gene family have been fully characterized. In this study, we report the identification of two novel U lineage genes isolated from the WIK strain of zebrafish. These new mhc1 genes, named una and uoa, are located in tandem on chromosome 19 with > 70% homology to previously isolated U genes. Sequencing of their neighboring genes revealed that una and uoa form a unique haplotype different from the previously known U lineage haplotypes. Additionally, we determined the expression profiles of U, Z, and L genes in three different tissues. These findings collectively suggest that mhc1 U lineage genes and their haplotypes in zebrafish are more divergent than previously considered, and their expression patterns vary significantly among different tissues.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping