PUBLICATION
Epitranscriptomic m6A regulation following spinal cord injury
- Authors
- Xing, L., Cai, Y., Yang, T., Yu, W., Gao, M., Chai, R., Ding, S., Wei, J., Pan, J., Chen, G.
- ID
- ZDB-PUB-201208-17
- Date
- 2020
- Source
- Journal of neuroscience research 99(3): 843-857 (Journal)
- Registered Authors
- Keywords
- Mettl3 (Methyltransferase Like 3), RNA methylation, RRID:AB_11212597, RRID:AB_2338072, RRID:AB_2338840, RRID:AB_2721254, RRID:AB_305313, m6A (N6-methyladenosine), spinal cord injury
- MeSH Terms
-
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Gene Expression Profiling
- Astrocytes
- Nerve Regeneration
- Adenosine/analogs & derivatives*
- Adenosine/genetics
- Neural Stem Cells
- Zebrafish
- Epigenesis, Genetic
- Male
- Mice, Inbred ICR
- Spinal Cord Injuries/metabolism*
- Animals
- Methyltransferases/metabolism*
- Methylation
- Mice
- PubMed
- 33271625 Full text @ J. Neurosci. Res.
Citation
Xing, L., Cai, Y., Yang, T., Yu, W., Gao, M., Chai, R., Ding, S., Wei, J., Pan, J., Chen, G. (2020) Epitranscriptomic m6A regulation following spinal cord injury. Journal of neuroscience research. 99(3):843-857.
Abstract
RNA methylation is involved in multiple physiological and pathological processes. However, the role of RNA methylation in spinal cord regeneration has not been reported. In this study, we find an altered m6A (N6-methyladenosine) RNA methylation profiling following zebrafish spinal cord injury (SCI), in line with an altered transcription level of the m6A methylase Mettl3. Interestingly, many of the differential m6A-tagged genes associated with neural regeneration are hypomethylated, but their transcription levels are upregulated in SCI. Moreover, we find that METTL3 may be important for spinal cord regeneration. We also show a conserved feature of METTL3 changes in mouse SCI model, in which the expression of METTL3 is increased in both astrocytes and neural stem cells. Together, our results indicate that m6A RNA methylation is dynamic and conserved following SCI and may contribute to spinal cord regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping