PUBLICATION

Epitranscriptomic m6A regulation following spinal cord injury

Authors
Xing, L., Cai, Y., Yang, T., Yu, W., Gao, M., Chai, R., Ding, S., Wei, J., Pan, J., Chen, G.
ID
ZDB-PUB-201208-17
Date
2020
Source
Journal of neuroscience research   99(3): 843-857 (Journal)
Registered Authors
Keywords
Mettl3 (Methyltransferase Like 3), RNA methylation, RRID:AB_11212597, RRID:AB_2338072, RRID:AB_2338840, RRID:AB_2721254, RRID:AB_305313, m6A (N6-methyladenosine), spinal cord injury
MeSH Terms
  • Epigenesis, Genetic
  • Methylation
  • Methyltransferases/metabolism*
  • Male
  • Adenosine/analogs & derivatives*
  • Adenosine/genetics
  • Gene Expression Profiling
  • Zebrafish
  • Neural Stem Cells
  • Mice
  • Spinal Cord Injuries/metabolism*
  • Mice, Inbred ICR
  • Astrocytes
  • Animals
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Nerve Regeneration
(all 17)
PubMed
33271625 Full text @ J. Neurosci. Res.
Abstract
RNA methylation is involved in multiple physiological and pathological processes. However, the role of RNA methylation in spinal cord regeneration has not been reported. In this study, we find an altered m6A (N6-methyladenosine) RNA methylation profiling following zebrafish spinal cord injury (SCI), in line with an altered transcription level of the m6A methylase Mettl3. Interestingly, many of the differential m6A-tagged genes associated with neural regeneration are hypomethylated, but their transcription levels are upregulated in SCI. Moreover, we find that METTL3 may be important for spinal cord regeneration. We also show a conserved feature of METTL3 changes in mouse SCI model, in which the expression of METTL3 is increased in both astrocytes and neural stem cells. Together, our results indicate that m6A RNA methylation is dynamic and conserved following SCI and may contribute to spinal cord regeneration.
Genes / Markers
Figures
No images available
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Xing, L., Cai, Y., Yang, T., Yu, W., Gao, M., Chai, R., Ding, S., Wei, J., Pan, J., Chen, G. (2020) Epitranscriptomic m6A regulation following spinal cord injury. Journal of neuroscience research. 99(3):843-857.