Pax9 is essential for granulopoiesis but dispensable for erythropoiesis in zebrafish
- Pak, B., Schmitt, C.E., Oh, S., Kim, J.D., Choi, W., Han, O., Kim, M., Kim, M.J., Ham, H.J., Kim, S., Huh, T.L., Kim, J.I., Jin, S.W.
- Biochemical and Biophysical Research Communications 534: 359-366 (Journal)
- Registered Authors
- Huh, Tae-Lin, Jin, Suk-Won, Kim, Jun-Dae
- Emergency granulopoiesis, Innate immunity, Paired box, Zebrafish, pax9
- MeSH Terms
- Animals, Genetically Modified
- Bacterial Infections/immunology
- CRISPR-Cas Systems
- Gene Expression Regulation, Developmental
- Gene Knockout Techniques
- Immunity, Innate/genetics
- Immunity, Innate/physiology
- PAX9 Transcription Factor/deficiency
- PAX9 Transcription Factor/genetics
- PAX9 Transcription Factor/immunology*
- Zebrafish Proteins/deficiency
- Zebrafish Proteins/genetics
- Zebrafish Proteins/immunology*
- 33256983 Full text @ Biochem. Biophys. Res. Commun.
Pak, B., Schmitt, C.E., Oh, S., Kim, J.D., Choi, W., Han, O., Kim, M., Kim, M.J., Ham, H.J., Kim, S., Huh, T.L., Kim, J.I., Jin, S.W. (2020) Pax9 is essential for granulopoiesis but dispensable for erythropoiesis in zebrafish. Biochemical and Biophysical Research Communications. 534:359-366.
Paired Box (Pax) gene family, a group of transcription regulators have been implicated in diverse physiological processes. However, their role during hematopoiesis which generate a plethora of blood cells remains largely unknown. Using a previously reported single cell transcriptomics data, we analyzed the expression of individual Pax family members in hematopoietic cells in zebrafish. We have identified that Pax9, which is an essential regulator for odontogenesis and palatogenesis, is selectively localized within a single cluster of the hematopoietic lineage. To further analyze the function of Pax9 in hematopoiesis, we generated two independent pax9 knock-out mutants using the CRISPR-Cas9 technique. We found that Pax9 appears to be an essential regulator for granulopoiesis but dispensable for erythropoiesis during development, as lack of pax9 selectively decreased the number of neutrophils with a concomitant decrease in the expression level of neutrophil markers. In addition, embryos, where pax9 was functionally disrupted by injecting morpholinos, failed to increase the number of neutrophils in response to pathogenic bacteria, suggesting that Pax9 is not only essential for developmental granulopoiesis but also emergency granulopoiesis. Due to the inability to initiate emergency granulopoiesis, innate immune responses were severely compromised in pax9 morpholino-mediated embryos, increasing their susceptibility and mortality. Taken together, our data indicate that Pax9 is essential for granulopoiesis and promotes innate immunity in zebrafish larvae.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes