PUBLICATION
Interactive effects of cadmium and Benzo[a]pyrene in adult zebrafish (Danio rerio) during short-term aqueous co-exposure
- Authors
- Kodzhahinchev, V., Shekh, K., Weber, L.P., Niyogi, S.
- ID
- ZDB-PUB-201130-11
- Date
- 2020
- Source
- Environmental pollution (Barking, Essex : 1987) 272: 116027 (Journal)
- Registered Authors
- Weber, Lynn
- Keywords
- Acute exposure, Benzo[a]Pyrene, Body burden, Cadmium, Gene expression, Mixture toxicity
- MeSH Terms
-
- Benzo(a)pyrene/toxicity
- Water Pollutants, Chemical*/toxicity
- Cadmium/toxicity
- Animals
- Zebrafish
- Polycyclic Aromatic Hydrocarbons*
- PubMed
- 33248835 Full text @ Environ. Pollut.
Citation
Kodzhahinchev, V., Shekh, K., Weber, L.P., Niyogi, S. (2020) Interactive effects of cadmium and Benzo[a]pyrene in adult zebrafish (Danio rerio) during short-term aqueous co-exposure. Environmental pollution (Barking, Essex : 1987). 272:116027.
Abstract
Environmental water quality guidelines often work under the assumption that the toxicity of environmental pollutants is identical when present in isolation or in a complex chemical mixture. Thus, there is a crucial gap in our knowledge regarding how these toxicants interact and alter the toxicological effects in aquatic organisms. The present study examined the effects of acute (72-hr) aqueous exposures of Cadmium (Cd), a highly toxic non-essential trace metal, and Benzo[a]Pyrene (B[a]P), a prototypical polycyclic aromatic hydrocarbon (PAH) in adult zebrafish. Following a range-finding series of individual single-toxicant exposures, a second series was carried out using select concentrations in binary mixture exposures (using 5.8 or 22 μg/L for Cd; 0.44 or 1.07 μg/L for B[a]P). Our results demonstrated that tissue accumulation of both toxicants increased significantly in the presence of the second toxicant relative to single-toxicant exposures. Cd-only and B[a]P-only single toxicant exposures caused a significant downregulation of cytochrome p4501a (CYP1A1) and metallothionein-2 (MT2) mRNA in the gills, respectively, however binary co-exposures using both toxicants resulted in strong up-regulation of CYP1A1 and MT2. Additionally, co-exposures caused a strong induction of SOD1 and CAT mRNA transcript levels in the gill. The observed increase in body burden and transcript modulation did not translate into additive or more-than-additive toxic effects (oxidative stress) in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping