PUBLICATION
A versatile and customizable low-cost 3D-printed open standard for microscopic imaging
- Authors
- Diederich, B., Lachmann, R., Carlstedt, S., Marsikova, B., Wang, H., Uwurukundo, X., Mosig, A.S., Heintzmann, R.
- ID
- ZDB-PUB-201127-2
- Date
- 2020
- Source
- Nature communications 11: 5979 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 33239615 Full text @ Nat. Commun.
Citation
Diederich, B., Lachmann, R., Carlstedt, S., Marsikova, B., Wang, H., Uwurukundo, X., Mosig, A.S., Heintzmann, R. (2020) A versatile and customizable low-cost 3D-printed open standard for microscopic imaging. Nature communications. 11:5979.
Abstract
Modern microscopes used for biological imaging often present themselves as black boxes whose precise operating principle remains unknown, and whose optical resolution and price seem to be in inverse proportion to each other. With UC2 (You. See. Too.) we present a low-cost, 3D-printed, open-source, modular microscopy toolbox and demonstrate its versatility by realizing a complete microscope development cycle from concept to experimental phase. The self-contained incubator-enclosed brightfield microscope monitors monocyte to macrophage cell differentiation for seven days at cellular resolution level (e.g. 2 μm). Furthermore, by including very few additional components, the geometry is transferred into a 400 Euro light sheet fluorescence microscope for volumetric observations of a transgenic Zebrafish expressing green fluorescent protein (GFP). With this, we aim to establish an open standard in optics to facilitate interfacing with various complementary platforms. By making the content and comprehensive documentation publicly available, the systems presented here lend themselves to easy and straightforward replications, modifications, and extensions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping