PUBLICATION
Morpho-functional remodelling of the adult zebrafish (Danio rerio) heart in response to waterborne angiotensin II exposure
- Authors
- Filice, M., Barca, A., Amelio, D., Leo, S., Mazzei, A., Vecchio, G.D., Verri, T., Cerra, M.C., Imbrogno, S.
- ID
- ZDB-PUB-201123-5
- Date
- 2020
- Source
- General and comparative endocrinology 301: 113663 (Journal)
- Registered Authors
- Keywords
- Angiotensin II, Angiotensin II receptors, Cardiac hypertrophy, Zebrafish
- MeSH Terms
-
- Angiotensin II
- Animals
- Heart
- Myocardium/metabolism
- Renin-Angiotensin System
- Zebrafish*
- PubMed
- 33220301 Full text @ Gen. Comp. Endocrinol.
Citation
Filice, M., Barca, A., Amelio, D., Leo, S., Mazzei, A., Vecchio, G.D., Verri, T., Cerra, M.C., Imbrogno, S. (2020) Morpho-functional remodelling of the adult zebrafish (Danio rerio) heart in response to waterborne angiotensin II exposure. General and comparative endocrinology. 301:113663.
Abstract
Angiotensin II (AngII), the principal effector of the Renin-Angiotensin System, is a pluripotent humoral agent whose biological actions include short-term modulations and long-term adaptations. In fish, short-term cardio-tropic effects of AngII are documented, but information on the role of AngII in long-term cardiac remodelling is not fully understood. Here, we describe a direct approach to disclose long-term morpho-functional effects of AngII on the zebrafish heart. Adult fish exposed to waterborne teleost analogue AngII for 8 weeks showed enhanced heart weight and cardio-somatic index, coupled to myocardial structural changes (i.e. augmented compacta thickness and fibrosis), and increased heart rate. These findings were paralleled by an up-regulation of type-1 and type-2 AngII receptors expression, and by changes in the expression of GATA binding protein 4, nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 and superoxide dismutase 1 soluble mRNAs, as well as of cytochrome b-245 beta polypeptide protein, indicative of cardiac remodelling. Our results suggest that waterborne AngII can sustain and robustly affect the cardiac morpho-functional remodelling of adult zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping