ZFIN ID: ZDB-PUB-201120-91
Spatial and Temporal Control of CRISPR-Cas9-Mediated Gene Editing Delivered via a Light-Triggered Liposome System
Aksoy, Y.A., Yang, B., Chen, W., Hung, T., Kuchel, R.P., Zammit, N.W., Grey, S.T., Goldys, E.M., Deng, W.
Date: 2020
Source: ACS applied materials & interfaces   12(47): 52433-52444 (Journal)
Registered Authors: Aksoy, Yagiz, Grey, Shane
Keywords: CRISPR-Cas9, light triggering, liposomes, protein release, temporal control
MeSH Terms:
  • Animals
  • CRISPR-Cas Systems/genetics*
  • Embryo, Nonmammalian/metabolism
  • Gene Editing/methods*
  • Gene Expression/drug effects
  • Genes, Reporter
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • HEK293 Cells
  • Humans
  • Light
  • Liposomes/chemistry*
  • Singlet Oxygen/metabolism
  • Tumor Necrosis Factor alpha-Induced Protein 3/genetics
  • Tumor Necrosis Factor alpha-Induced Protein 3/metabolism
  • Tumor Necrosis Factor-alpha/pharmacology
  • Zebrafish/growth & development
  • Zebrafish/metabolism
PubMed: 33174413 Full text @ ACS Appl. Mater. Interfaces
ABSTRACT
The CRISPR-Cas9 and related systems offer a unique genome-editing tool allowing facile and efficient introduction of heritable and locus-specific sequence modifications in the genome. Despite its molecular precision, temporal and spatial control of gene editing with the CRISPR-Cas9 system is very limited. We developed a light-sensitive liposome delivery system that offers a high degree of spatial and temporal control of gene editing with the CRISPR-Cas9 system. We demonstrated its efficient protein release by respectively assessing the targeted knockout of the eGFP gene in human HEK293/GFP cells and the TNFAIP3 gene in TNFα-induced HEK293 cells. We further validated our results at a single-cell resolution using an in vivo eGFP reporter system in zebrafish (77% knockout). These findings indicate that light-triggered liposomes may have new options for precise control of CRISPR-Cas9 release and editing.
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