|ZFIN ID: ZDB-PUB-201120-91|
Spatial and Temporal Control of CRISPR-Cas9-Mediated Gene Editing Delivered via a Light-Triggered Liposome System
Aksoy, Y.A., Yang, B., Chen, W., Hung, T., Kuchel, R.P., Zammit, N.W., Grey, S.T., Goldys, E.M., Deng, W.
|Source:||ACS applied materials & interfaces 12(47): 52433-52444 (Journal)|
|Registered Authors:||Aksoy, Yagiz, Grey, Shane|
|Keywords:||CRISPR-Cas9, light triggering, liposomes, protein release, temporal control|
|PubMed:||33174413 Full text @ ACS Appl. Mater. Interfaces|
Aksoy, Y.A., Yang, B., Chen, W., Hung, T., Kuchel, R.P., Zammit, N.W., Grey, S.T., Goldys, E.M., Deng, W. (2020) Spatial and Temporal Control of CRISPR-Cas9-Mediated Gene Editing Delivered via a Light-Triggered Liposome System. ACS applied materials & interfaces. 12(47):52433-52444.
ABSTRACTThe CRISPR-Cas9 and related systems offer a unique genome-editing tool allowing facile and efficient introduction of heritable and locus-specific sequence modifications in the genome. Despite its molecular precision, temporal and spatial control of gene editing with the CRISPR-Cas9 system is very limited. We developed a light-sensitive liposome delivery system that offers a high degree of spatial and temporal control of gene editing with the CRISPR-Cas9 system. We demonstrated its efficient protein release by respectively assessing the targeted knockout of the eGFP gene in human HEK293/GFP cells and the TNFAIP3 gene in TNFα-induced HEK293 cells. We further validated our results at a single-cell resolution using an in vivo eGFP reporter system in zebrafish (77% knockout). These findings indicate that light-triggered liposomes may have new options for precise control of CRISPR-Cas9 release and editing.