PUBLICATION
In vivo hepatotoxicity screening of different extracts, components, and constituents of Polygoni Multiflori Thunb. in zebrafish (Danio rerio) larvae
- Authors
- Li, H.Y., Yang, J.B., Li, W.F., Qiu, C.X., Hu, G., Wang, S.T., Song, Y.F., Gao, H.Y., Liu, Y., Wang, Q., Wang, Y., Cheng, X.L., Wei, F., Jin, H.T., Ma, S.C.
- ID
- ZDB-PUB-201120-39
- Date
- 2020
- Source
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 131: 110524 (Journal)
- Registered Authors
- Keywords
- Chrysophanol, Dianthrone, Emodin, Emodin-8-O-β-D-glucopyranoside, Zebrafish hepatotoxicity model, [Trans]-emodin-emodin dianthrone
- MeSH Terms
-
- Animals
- Chemical and Drug Induced Liver Injury*
- Drug Evaluation, Preclinical
- Emodin/toxicity
- Fallopia multiflora/chemistry*
- Larva/drug effects
- Medicine, Chinese Traditional
- Plant Extracts/toxicity*
- Polyphenols/toxicity
- Zebrafish/embryology
- PubMed
- 33152900 Full text @ Biomed. Pharmacother.
Citation
Li, H.Y., Yang, J.B., Li, W.F., Qiu, C.X., Hu, G., Wang, S.T., Song, Y.F., Gao, H.Y., Liu, Y., Wang, Q., Wang, Y., Cheng, X.L., Wei, F., Jin, H.T., Ma, S.C. (2020) In vivo hepatotoxicity screening of different extracts, components, and constituents of Polygoni Multiflori Thunb. in zebrafish (Danio rerio) larvae. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 131:110524.
Abstract
Polygonum multiflorum Thunb. (PM) is a traditional Chinese medicine, commonly used to treat a variety of diseases. However, the hepatotoxicity associated with PM hampers its clinical application and development. In this study, we refined the zebrafish hepatotoxicity model with regard to the following endpoints: liver size, liver gray value, and the area of yolk sac. The levels of alanine aminotransferase, aspartate transaminase, albumin, and microRNAs-122 were evaluated to verify the model. Subsequently, this model was used to screen different extracts, components, and constituents of PM, including 70 % EtOH extracts of PM, four fractions from macroporous resin (components A, B, C, and D), and 19 compounds from component D. We found that emodin, chrysophanol, emodin-8-O-β-D-glucopyranoside, (cis)-emodin-emodin dianthrones, and (trans)-emodin-emodin dianthrones showed higher hepatotoxicity compared to other components in PM, whereas polyphenols showed lower hepatotoxicity. To the best of our knowledge, this study is the first to identify that dianthrones may account for the hepatotoxicity of PM. We believe that these findings will be helpful in regulating the hepatotoxicity of PM.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping