PUBLICATION
Fast in vivo multiphoton light-sheet microscopy with optimal pulse frequency
- Authors
- Maioli, V., Boniface, A., Mahou, P., Ortas, J.F., Abdeladim, L., Beaurepaire, E., Supatto, W.
- ID
- ZDB-PUB-201120-34
- Date
- 2020
- Source
- Biomedical Optics Express 11: 6012-6026 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 33150002 Full text @ Biomed. Opt. Express
Citation
Maioli, V., Boniface, A., Mahou, P., Ortas, J.F., Abdeladim, L., Beaurepaire, E., Supatto, W. (2020) Fast in vivo multiphoton light-sheet microscopy with optimal pulse frequency. Biomedical Optics Express. 11:6012-6026.
Abstract
Improving the imaging speed of multiphoton microscopy is an active research field. Among recent strategies, light-sheet illumination holds distinctive advantages for achieving fast imaging in vivo. However, photoperturbation in multiphoton light-sheet microscopy remains poorly investigated. We show here that the heart beat rate of zebrafish embryos is a sensitive probe of linear and nonlinear photoperturbations. By analyzing its behavior with respect to laser power, pulse frequency and wavelength, we derive guidelines to find the best balance between signal and photoperturbation. We then demonstrate one order-of-magnitude signal enhancement over previous implementations by optimizing the laser pulse frequency. These results open new opportunities for fast live tissue imaging.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping