PUBLICATION
Tissue-Specific In Vivo Biotin Chromatin Immunoprecipitation with Sequencing in Zebrafish and Chicken
- Authors
- Lukoseviciute, M., Ling, I.T.C., Senanayake, U., Candido-Ferreira, I., Taylor, G., Williams, R.M., Sauka-Spengler, T.
- ID
- ZDB-PUB-201029-6
- Date
- 2020
- Source
- STAR protocols 1: 100066 (Journal)
- Registered Authors
- Sauka-Spengler, Tatjana
- Keywords
- none
- Datasets
- GEO:GSE106676
- MeSH Terms
-
- Animals
- Biotin/chemistry*
- Biotin/metabolism
- Cells, Cultured
- Chickens/genetics
- Chromatin Immunoprecipitation/methods*
- Organ Specificity/physiology
- Sequence Analysis, DNA/methods*
- Streptavidin/chemistry
- Streptavidin/metabolism
- Transcription Factors/chemistry
- Transcription Factors/metabolism
- Zebrafish/genetics
- PubMed
- 33111104 Full text @ STAR Protoc
Citation
Lukoseviciute, M., Ling, I.T.C., Senanayake, U., Candido-Ferreira, I., Taylor, G., Williams, R.M., Sauka-Spengler, T. (2020) Tissue-Specific In Vivo Biotin Chromatin Immunoprecipitation with Sequencing in Zebrafish and Chicken. STAR protocols. 1:100066.
Abstract
Chromatin immunoprecipitation with sequencing (ChIP-seq) has been instrumental in understanding transcription factor (TF) binding during gene regulation. ChIP-seq requires specific antibodies against desired TFs, which are not available for numerous species. Here, we describe a tissue-specific biotin ChIP-seq protocol for zebrafish and chicken embryos which utilizes AVI tagging of TFs, permitting their biotinylation by a co-expressed nuclear biotin ligase. Subsequently, biotinylated factors can be precipitated with streptavidin beads, enabling the user to construct TF genome-wide binding landscapes like conventional ChIP-seq methods. For complete details on the use and execution of this protocol, please see Lukoseviciute et al. (2018) and Ling and Sauka-Spengler (2019).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping