PUBLICATION
E-cadherin focuses protrusion formation at the front of migrating cells by impeding actin flow
- Authors
- Grimaldi, C., Schumacher, I., Boquet-Pujadas, A., Tarbashevich, K., Vos, B.E., Bandemer, J., Schick, J., Aalto, A., Olivo-Marin, J.C., Betz, T., Raz, E.
- ID
- ZDB-PUB-201028-4
- Date
- 2020
- Source
- Nature communications 11: 5397 (Journal)
- Registered Authors
- Aalto, Anne, Bandemer, Jan, Grimaldi, Cecilia, Raz, Erez, Tarbashevich, Katsiyarina
- Keywords
- none
- MeSH Terms
-
- Actins/genetics
- Actins/metabolism*
- Actomyosin/genetics
- Actomyosin/metabolism
- Animals
- Cadherins/genetics
- Cadherins/metabolism*
- Cell Movement*
- Female
- Germ Cells/cytology*
- Germ Cells/metabolism
- Male
- Pseudopodia/genetics
- Pseudopodia/metabolism*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 33106478 Full text @ Nat. Commun.
Citation
Grimaldi, C., Schumacher, I., Boquet-Pujadas, A., Tarbashevich, K., Vos, B.E., Bandemer, J., Schick, J., Aalto, A., Olivo-Marin, J.C., Betz, T., Raz, E. (2020) E-cadherin focuses protrusion formation at the front of migrating cells by impeding actin flow. Nature communications. 11:5397.
Abstract
The migration of many cell types relies on the formation of actomyosin-dependent protrusions called blebs, but the mechanisms responsible for focusing this kind of protrusive activity to the cell front are largely unknown. Here, we employ zebrafish primordial germ cells (PGCs) as a model to study the role of cell-cell adhesion in bleb-driven single-cell migration in vivo. Utilizing a range of genetic, reverse genetic and mathematical tools, we define a previously unknown role for E-cadherin in confining bleb-type protrusions to the leading edge of the cell. We show that E-cadherin-mediated frictional forces impede the backwards flow of actomyosin-rich structures that define the domain where protrusions are preferentially generated. In this way, E-cadherin confines the bleb-forming region to a restricted area at the cell front and reinforces the front-rear axis of migrating cells. Accordingly, when E-cadherin activity is reduced, the bleb-forming area expands, thus compromising the directional persistence of the cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
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