PUBLICATION

Hedgehog signaling regulates neurogenesis in the larval and adult zebrafish hypothalamus

Authors
Male, I., Ozacar, A.T., Fagan, R.R., Loring, M.D., Loring, M.D., Shen, M.C., Pace, V.A., Devine, C.A., Lawson, G.E., Lutservitz, A., Karlstrom, R.O.
ID
ZDB-PUB-201028-3
Date
2020
Source
eNeuro   7(6): (Journal)
Registered Authors
Karlstrom, Rolf, Ozacar, Ayse Tuba
Keywords
Hedgehog, Hypothalamus, Neurogenesis, Zebrafish
MeSH Terms
  • Hedgehog Proteins*/genetics
  • Hedgehog Proteins*/metabolism
  • Animals
  • Hypothalamus/metabolism
  • Zebrafish*/metabolism
  • Signal Transduction
  • Neurogenesis
  • Larva/metabolism
(all 8)
PubMed
33106384 Full text @ eNeuro
Abstract
Neurogenesis is now known to play a role in adult hypothalamic function, yet the cell-cell mechanisms regulating this neurogenesis remain poorly understood. Here we show that Hedgehog/Gli signaling positively regulates hypothalamic neurogenesis in both larval and adult zebrafish and is necessary and sufficient for normal hypothalamic proliferation rates. Hedgehog-responsive radial glia represent a relatively highly proliferative precursor population that gives rise to dopaminergic, serotonergic, and GABAergic neurons. in situ and transgenic reporter analyses revealed substantial heterogeneity in cell-cell signaling within the hypothalamic niche, with slow cycling Nestin-expressing cells residing among distinct and overlapping populations of Sonic Hh (Shh)-expressing, Hh-responsive, Notch-responsive, and Wnt-responsive radial glia. This work shows for the first time that Hh/Gli-signaling is a key component of the complex cell-cell signaling environment that regulates hypothalamic neurogenesis throughout life.Significance statement The extent, control, and consequences of adult neurogenesis in the hypothalamus are not well understood, despite the critical integrative role this conserved brain region plays in regulating basic metabolic and reproductive functions across vertebrate species. Here we show that proliferation in the zebrafish hypothalamus continues into adulthood and begin to define the complex signaling environment of the hypothalamic niche that may regulate this adult neurogenesis. Using new conditional gene regulation tools we show that the evolutionarily conserved Hedgehog/Gli signaling pathway positively regulates hypothalamic neurogenesis during postembryonic development and into adulthood. These studies suggest a mechanism for the control of hypothalamic growth and tissue renewal, as well as the plasticity in neuroendocrine cell populations that is now linked to hypothalamic function.
Genes / Markers
Figures
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a4596TgTransgenic Insertion
    ia4TgTransgenic Insertion
      nns9TgTransgenic Insertion
        nns25TgTransgenic Insertion
          ot80TgTransgenic Insertion
            scz032TgTransgenic Insertion
              t10TgTransgenic Insertion
                tud101TgTransgenic Insertion
                  um14TgTransgenic Insertion
                    umz23TgTransgenic Insertion
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                      Human Disease / Model
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                      Sequence Targeting Reagents
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                      Fish
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                      Antibodies
                      Orthology
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                      Engineered Foreign Genes
                      Marker Marker Type Name
                      CreEFGCre
                      DsRedEFGDsRed
                      EGFPEFGEGFP
                      GFPEFGGFP
                      KaedeEFGKaede
                      mCherryEFGmCherry
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                      Citation
                      Male, I., Ozacar, A.T., Fagan, R.R., Loring, M.D., Loring, M.D., Shen, M.C., Pace, V.A., Devine, C.A., Lawson, G.E., Lutservitz, A., Karlstrom, R.O. (2020) Hedgehog signaling regulates neurogenesis in the larval and adult zebrafish hypothalamus. eNeuro. 7(6):.