PUBLICATION
Visualization of extracellular vesicles in the regenerating caudal fin blastema of zebrafish using in vivo electroporation
- Authors
- Ohgo, S., Sakamoto, T., Nakajima, W., Matsunaga, S., Wada, N.
- ID
- ZDB-PUB-201021-9
- Date
- 2020
- Source
- Biochemical and Biophysical Research Communications 533(4): 1371-1377 (Journal)
- Registered Authors
- Keywords
- Blastema, Extracellular vesicle, Regeneration, Zebrafish
- MeSH Terms
-
- Animal Fins/cytology
- Animal Fins/physiology*
- Animals
- Animals, Genetically Modified
- Electroporation/methods*
- Extracellular Vesicles*/metabolism
- Gene Transfer Techniques
- Microscopy, Fluorescence/instrumentation
- Molecular Biology/instrumentation
- Molecular Biology/methods
- Plasmids/administration & dosage
- Plasmids/genetics
- Regeneration/physiology*
- Tetraspanin 30/genetics
- Zebrafish/physiology*
- Zebrafish Proteins/genetics
- PubMed
- 33077180 Full text @ Biochem. Biophys. Res. Commun.
Citation
Ohgo, S., Sakamoto, T., Nakajima, W., Matsunaga, S., Wada, N. (2020) Visualization of extracellular vesicles in the regenerating caudal fin blastema of zebrafish using in vivo electroporation. Biochemical and Biophysical Research Communications. 533(4):1371-1377.
Abstract
Zebrafish have high regenerative ability in several organs including the fin. Although various mechanisms underlying fin regeneration have been revealed, some mechanisms remain to be elucidated. Recently, extracellular vesicles (EVs) have been the focus of research with regard to their role in cell-to-cell communication. It has been suggested that cells in regenerating tissues communicate using EVs. In this study, we examined the involvement of EVs in the caudal fin regeneration of zebrafish using an in vivo electroporation method. The process of regeneration appeared normal after in vivo electroporation, and the transferred plasmid showed mosaic expression in the blastema. We took advantage of this mosaic expression to observe the distribution of exosomal markers in the blastema. We transferred exosomal markers by in vivo electroporation and identified EVs in the regenerating caudal fin. The results suggest that blastemal cells communicate with other cells via EVs during caudal fin regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping