PUBLICATION
A comparative study of the expression patterns of Fign family members in zebrafish embryonic development
- Authors
- Dong, Z., Li, Y., Chen, X., Lai, X., Liu, M.
- ID
- ZDB-PUB-201020-56
- Date
- 2020
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 251: 110522 (Journal)
- Registered Authors
- Keywords
- Fidgetin like 1 (fignl1), Fidgetin like 2 (fignl2), Fign, In situ hybridization (ISH), Zebrafish embryonic development
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Computational Biology
- Embryonic Development/genetics*
- Gene Expression Regulation, Developmental*
- Microtubule-Associated Proteins/chemistry
- Microtubule-Associated Proteins/genetics*
- Microtubule-Associated Proteins/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics*
- PubMed
- 33069857 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Dong, Z., Li, Y., Chen, X., Lai, X., Liu, M. (2020) A comparative study of the expression patterns of Fign family members in zebrafish embryonic development. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 251:110522.
Abstract
During development, highly dynamic reconstruction of microtubules is involved in many cellular processes, including cell division, migration, morphological changes, and material transportation within cells. Microtubule severing proteins (MSPs), with the function of cutting microtubules into short parts, are important regulators in the reconstruction of microtubule arrays. Fidgetin (fign) and its family members fidgetin like 1 (fignl1) and fignl2 are MSPs, and knowledge on the expression patterns of fign family members will benefit our understanding of their primary roles in one specific stage during development. In this study, we compared the evolutionary relationships of fign family members and found that fignl2 is closer to fign than fignl1. We utilized the zebrafish model and in situ hybridization (ISH) to parallelly identify the expression features of fign family members. Our findings revealed that before 12 h post fertilization (hpf), the expression patterns of fign and fignl1 and fignl2 genes were similar, but differences arose thereafter. Fignl2 transcripts were present in more tissues and organs of zebrafish after 12 hpf and potentially exhibited more ubiquitous functions. This study is the first to assess systematic comparable data on the expression patterns of fign family members during development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping