PUBLICATION
A novel chemical-combination screen in zebrafish identifies epigenetic small molecule candidates for the treatment of Duchenne muscular dystrophy
- Authors
- Farr, G.H., Morris, M., Gomez, A., Pham, T., Kilroy, E., Parker, E.U., Said, S., Henry, C., Maves, L.
- ID
- ZDB-PUB-201020-22
- Date
- 2020
- Source
- Skeletal muscle 10: 29 (Journal)
- Registered Authors
- Farr III, G. Hank, Henry, Clarissa A., Maves, Lisa
- Keywords
- Chemical screen, Duchenne muscular dystrophy, Epigenetic small molecules, HDAC inhibitors, Zebrafish
- MeSH Terms
-
- Animals
- Cells, Cultured
- Drug Discovery
- Epigenesis, Genetic
- High-Throughput Screening Assays
- Histone Deacetylase Inhibitors/pharmacology*
- Hydroxamic Acids/pharmacology
- Membrane Proteins/genetics
- Muscle Proteins/genetics
- Muscle, Skeletal/drug effects*
- Muscle, Skeletal/metabolism
- Muscular Dystrophy, Duchenne/drug therapy*
- Muscular Dystrophy, Duchenne/genetics
- Naphthols/pharmacology
- Phenylpropionates/pharmacology
- Small Molecule Libraries/pharmacology*
- Zebrafish
- Zebrafish Proteins/genetics
- PubMed
- 33059738 Full text @ Skelet Muscle
Citation
Farr, G.H., Morris, M., Gomez, A., Pham, T., Kilroy, E., Parker, E.U., Said, S., Henry, C., Maves, L. (2020) A novel chemical-combination screen in zebrafish identifies epigenetic small molecule candidates for the treatment of Duchenne muscular dystrophy. Skeletal muscle. 10:29.
Abstract
Background Duchenne muscular dystrophy (DMD) is a severe neuromuscular disorder and is one of the most common muscular dystrophies. There are currently few effective therapies to treat the disease, although many small-molecule approaches are being pursued. Certain histone deacetylase inhibitors (HDACi) have been shown to ameliorate DMD phenotypes in mouse and zebrafish animal models. The HDACi givinostat has shown promise for DMD in clinical trials. However, beyond a small group of HDACi, other classes of epigenetic small molecules have not been broadly and systematically studied for their benefits for DMD.
Methods We used an established animal model for DMD, the zebrafish dmd mutant strain sapje. A commercially available library of epigenetic small molecules was used to treat embryonic-larval stages of dmd mutant zebrafish. We used a quantitative muscle birefringence assay in order to assess and compare the effects of small-molecule treatments on dmd mutant zebrafish skeletal muscle structure.
Results We performed a novel chemical-combination screen of a library of epigenetic compounds using the zebrafish dmd model. We identified candidate pools of epigenetic compounds that improve skeletal muscle structure in dmd mutant zebrafish. We then identified a specific combination of two HDACi compounds, oxamflatin and salermide, that ameliorated dmd mutant zebrafish skeletal muscle degeneration. We validated the effects of oxamflatin and salermide on dmd mutant zebrafish in an independent laboratory. Furthermore, we showed that the combination of oxamflatin and salermide caused increased levels of histone H4 acetylation in zebrafish larvae.
Conclusions Our results provide novel, effective methods for performing a combination of small-molecule screen in zebrafish. Our results also add to the growing evidence that epigenetic small molecules may be promising candidates for treating DMD.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping