PUBLICATION

Adrenergic activation modulates the signal from the Reissner fiber to cerebrospinal fluid-contacting neurons during development

Authors
Cantaut-Belarif, Y., Orts Del'Immagine, A., Penru, M., Pézeron, G., Wyart, C., Bardet, P.L.
ID
ZDB-PUB-201015-14
Date
2020
Source
eLIFE   9: (Journal)
Registered Authors
Bardet, Pierre-Luc, Pézeron, Guillaume, Wyart, Claire
Keywords
developmental biology, neuroscience, zebrafish
MeSH Terms
  • Animals
  • Cerebrospinal Fluid/metabolism*
  • Embryo, Nonmammalian/embryology
  • Fish Proteins/genetics
  • Fish Proteins/metabolism
  • Gene Expression
  • Morphogenesis/physiology
  • Neurons/physiology*
  • Neuropeptides/genetics
  • Neuropeptides/metabolism
  • Signal Transduction
  • Spinal Nerves/embryology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed
33048048 Full text @ Elife
Abstract
The cerebrospinal fluid (CSF) contains an extracellular thread conserved in vertebrates, the Reissner fiber, which controls body axis morphogenesis in the zebrafish embryo. Yet, the signaling cascade originating from this fiber to ensure body axis straightening is not understood. Here, we explore the functional link between the Reissner fiber and undifferentiated spinal neurons contacting the CSF (CSF-cNs). First, we show that the Reissner fiber is required in vivo for the expression of urp2, a neuropeptide expressed in CSF-cNs. We show that the Reissner fiber is also required for embryonic calcium transients in these spinal neurons. Finally, we study how local adrenergic activation can substitute for the Reissner fiber-signaling pathway to CSF-cNs and rescue body axis morphogenesis. Our results show that the Reissner fiber acts on CSF-cNs and thereby contributes to establish body axis morphogenesis, and suggest it does so by controlling the availability of a chemical signal in the CSF.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes