PUBLICATION
Biomass-derived cellulose nanoparticles display considerable neurotoxicity in zebrafish
- Authors
- Liu, C., Zhao, J., Zhang, X., Wei, G., Hao, W., Wang, X., Yang, C., Shi, Y., Liu, D.
- ID
- ZDB-PUB-201015-10
- Date
- 2020
- Source
- International journal of biological macromolecules 165(Pt B): 1783-1792 (Journal)
- Registered Authors
- Keywords
- Cellulose nanoparticles, Neuron, Toxicity, Transcriptome sequencing, Zebrafish
- MeSH Terms
-
- Animals
- Biomass*
- Cellulose/toxicity*
- Embryo, Nonmammalian/blood supply
- Embryo, Nonmammalian/drug effects
- Gene Ontology
- Larva/drug effects
- Motor Activity/drug effects
- Motor Neurons/drug effects
- Motor Neurons/pathology
- Nanofibers/toxicity
- Nanoparticles/toxicity*
- Neurotoxins/toxicity*
- Particle Size
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Toxicity Tests*
- Zebrafish/embryology
- Zebrafish/physiology*
- PubMed
- 33045296 Full text @ Int. J. Biol. Macromol.
Citation
Liu, C., Zhao, J., Zhang, X., Wei, G., Hao, W., Wang, X., Yang, C., Shi, Y., Liu, D. (2020) Biomass-derived cellulose nanoparticles display considerable neurotoxicity in zebrafish. International journal of biological macromolecules. 165(Pt B):1783-1792.
Abstract
The widespread use of nanomaterials poses a great threat to human living environments. Among them, biomass-derived cellulose nanoparticle (CN) is one of the widely used nanomaterials. To date, the toxicity of CNs during embryonic development remains undetermined. In this study, we exposed zebrafish embryos to cellulose nanofibrils (CNFs) and cellulose nanocrystals (CNCs) to evaluate the toxicity of these CNs. Exposure to CNFs or CNCs below 30 mg/ml exhibited no dose-dependent increases in malformation and mortality in zebrafish embryos. Then we demonstrated that CNs were highly enriched in zebrafish embryo via imaging analyses of embryos treated with FITC-coupled CNCs. In addition, we found that CNF or CNC exposure resulted in compromised motor ability of zebrafish larva. Furthermore, it was revealed that the differentiation and the morphogenesis of motor neurons were significantly interrupted. While, blood vessels were normally patterned, suggesting the specific neurotoxicity of these nanomaterials. Transcriptome sequencing assay showed that the neurotoxicity of CNs in the motor neurons might be attributed to the expression alteration of neural genes. In summary, we discovered the neurotoxicity of CNs for the first time.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping