ZFIN ID: ZDB-PUB-201008-13
Endoderm-Derived Myeloid-like Metaphocytes in Zebrafish Gill Mediate Soluble Antigen-Induced Immunity
Lin, X., Zhou, Q., Lin, G., Zhao, C., Wen, Z.
Date: 2020
Source: Cell Reports   33: 108227 (Journal)
Registered Authors: Wen, Zilong
Keywords: endoderm, gill, intestine, macrophage, metaphocyte, zebrafish
Microarrays: GEO:GSE155287
MeSH Terms:
  • Animals
  • Endoderm/metabolism*
  • Gills
  • Zebrafish
  • Zebrafish Proteins/metabolism*
PubMed: 33027664 Full text @ Cell Rep.
Immune cells in the mucosal barriers of vertebrates are highly heterogeneous in their origin and function. This heterogeneity is further exemplified by the recent discovery of ectoderm-derived immune cells-metaphocytes in zebrafish epidermis. Yet, whether non-hematopoiesis-derived immune cells generally exist in barrier tissues remains obscured. Here, we report the identification and characterization of an endoderm-derived immune cell population in the gill and intestine of zebrafish. Transcriptome analysis reveals that the endoderm-derived immune cells are myeloid-like cells with high similarities to the ectoderm-derived metaphocytes in epidermis. Like metaphocytes in epidermis, the endoderm-derived immune cells are non-phagocytic but professional in external soluble antigen uptake. Depletion of the endoderm-derived immune cells in gill hinder the local immune response to external soluble stimulants. This study demonstrates a general existence of non-hematopoiesis-derived immune cells in zebrafish mucosal barriers and challenges the prevalent view that resident immune cells in mucosal barriers arise exclusively from hematopoiesis.