PUBLICATION
Gene regulatory networks controlling vertebrate retinal regeneration
- Authors
- Hoang, T., Wang, J., Boyd, P., Wang, F., Santiago, C., Jiang, L., Yoo, S., Lahne, M., Todd, L.J., Jia, M., Saez, C., Keuthan, C., Palazzo, I., Squires, N., Campbell, W.A., Rajaii, F., Parayil, T., Trinh, V., Kim, D.W., Wang, G., Campbell, L.J., Ash, J., Fischer, A.J., Hyde, D.R., Qian, J., Blackshaw, S.
- ID
- ZDB-PUB-201003-6
- Date
- 2020
- Source
- Science (New York, N.Y.) 370(6519): (Journal)
- Registered Authors
- Keywords
- none
- Datasets
- GEO:GSE135406
- MeSH Terms
-
- Animals
- Cellular Reprogramming/genetics*
- Chickens
- Ependymoglial Cells/cytology*
- Gene Expression Regulation, Developmental
- Gene Regulatory Networks*
- Mice
- Nerve Regeneration/genetics*
- Neurogenesis/genetics*
- RNA-Seq
- Zebrafish
- PubMed
- 33004674 Full text @ Science
Citation
Hoang, T., Wang, J., Boyd, P., Wang, F., Santiago, C., Jiang, L., Yoo, S., Lahne, M., Todd, L.J., Jia, M., Saez, C., Keuthan, C., Palazzo, I., Squires, N., Campbell, W.A., Rajaii, F., Parayil, T., Trinh, V., Kim, D.W., Wang, G., Campbell, L.J., Ash, J., Fischer, A.J., Hyde, D.R., Qian, J., Blackshaw, S. (2020) Gene regulatory networks controlling vertebrate retinal regeneration. Science (New York, N.Y.). 370(6519):.
Abstract
Injury induces retinal Müller glia of certain cold-blooded vertebrates, but not mammals, to regenerate neurons. To identify gene regulatory networks that reprogram Müller glia into progenitor cells, we profiled changes in gene expression and chromatin accessibility in Müller glia from zebrafish, chick and mice in response to different stimuli. We identified evolutionarily conserved and species-specific gene networks controlling glial quiescence, reactivity and neurogenesis. In zebrafish and chick, transition from the quiescence to reactivity is essential for retinal regeneration, while in mice a dedicated network suppresses neurogenic competence and restores quiescence. Disruption of nuclear factor I (NFI) transcription factors, which maintain and restore quiescence, induces Müller glia to proliferate and generate neurons in adult mice following injury. These findings may aid in designing therapies to restore retinal neurons lost to degenerative diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping