PUBLICATION
PLK1- and PLK4-Mediated Asymmetric Mitotic Centrosome Size and Positioning in the Early Zebrafish Embryo
- Authors
- Rathbun, L.I., Aljiboury, A.A., Bai, X., Hall, N.A., Manikas, J., Amack, J.D., Bembenek, J.N., Hehnly, H.
- ID
- ZDB-PUB-201002-25
- Date
- 2020
- Source
- Current biology : CB 30(22): 4519-4527.e3 (Journal)
- Registered Authors
- Amack, Jeffrey, Hehnly, Heidi
- Keywords
- C. elegans, PCM, PLK1, PLK4, centrosome, embryo, microtubules, mitosis, spindle, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Caenorhabditis elegans
- Cell Cycle Proteins/genetics
- Cell Cycle Proteins/metabolism*
- Cell Size
- Centrosome/metabolism*
- Embryo, Nonmammalian
- Embryonic Development*
- Intravital Microscopy
- Microscopy, Confocal
- Mitosis
- Protein Serine-Threonine Kinases/genetics
- Protein Serine-Threonine Kinases/metabolism*
- Proto-Oncogene Proteins/genetics
- Proto-Oncogene Proteins/metabolism*
- Spindle Apparatus/metabolism*
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 32916112 Full text @ Curr. Biol.
Citation
Rathbun, L.I., Aljiboury, A.A., Bai, X., Hall, N.A., Manikas, J., Amack, J.D., Bembenek, J.N., Hehnly, H. (2020) PLK1- and PLK4-Mediated Asymmetric Mitotic Centrosome Size and Positioning in the Early Zebrafish Embryo. Current biology : CB. 30(22):4519-4527.e3.
Abstract
Factors that regulate mitotic spindle positioning remain unclear within the confines of extremely large embryonic cells, such as the early divisions of the vertebrate embryo, Danio rerio (zebrafish). We find that the mitotic centrosome, a structure that assembles the mitotic spindle [1], is notably large in the zebrafish embryo (246.44 ± 11.93 μm2 in a 126.86 ± 0.35 μm diameter cell) compared to a C. elegans embryo (5.78 ± 0.18 μm2 in a 55.83 ± 1.04 μm diameter cell). During embryonic cell divisions, cell size changes rapidly in both C. elegans and zebrafish [2, 3], where mitotic centrosome area scales more closely with changes in cell size compared to changes in spindle length. Embryonic zebrafish spindles contain asymmetrically sized mitotic centrosomes (2.14 ± 0.13-fold difference between the two), with the larger mitotic centrosome placed toward the embryo center in a polo-like kinase (PLK) 1- and PLK4-dependent manner. We propose a model in which uniquely large zebrafish embryonic centrosomes direct spindle placement within disproportionately large cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping