PUBLICATION

The low affinity p75 neurotrophin receptor is down-regulated in congenital anomalies of the kidney and the urinary tract: Possible involvement in early nephrogenesis

Authors
Fédou, C., Lescat, O., Feuillet, G., Buléon, M., Neau, E., Breuil, B., Alvès, M., Batut, J., Blader, P., Decramer, S., Saulnier-Blache, J.S., Klein, J., Buffin-Meyer, B., Schanstra, J.P.
ID
ZDB-PUB-201002-200
Date
2020
Source
Biochemical and Biophysical Research Communications   533(4): 786-791 (Journal)
Registered Authors
Batut, Julie, Blader, Patrick
Keywords
CAKUT, Nephrogenesis, Zebrafish, p75NTR
MeSH Terms
  • Animals
  • Down-Regulation
  • Humans
  • Kidney/abnormalities*
  • Kidney/embryology*
  • Kidney/metabolism
  • Mice
  • Nerve Tissue Proteins/metabolism*
  • Pronephros
  • RNA, Messenger/metabolism
  • Receptors, Nerve Growth Factor/genetics
  • Receptors, Nerve Growth Factor/metabolism*
  • Urinary Tract/abnormalities*
  • Zebrafish/embryology
PubMed
32988586 Full text @ Biochem. Biophys. Res. Commun.
Abstract
Congenital Anomalies of the Kidney and of the Urinary Tract (CAKUT) cover a broad range of disorders including abnormal kidney development caused by defective nephrogenesis. Here we explored the possible involvement of the low affinity p75 neurotrophin receptor (p75NTR) in CAKUT and nephrogenesis. In mouse, p75NTR was highly expressed in fetal kidney, located within cortical early nephrogenic bodies, and decreased rapidly after birth. In human control fetal kidney, p75NTR was also located within the early nephrogenic bodies as well as in the mature glomeruli, presumably in the mesangium. In CAKUT fetal kidneys, the kidney cortical structure and the localization of p75NTR were often disorganized, and quantification of p75NTR in amniotic fluid revealed a significant reduction in CAKUT compared to control. Finally, invalidation of p75NTR in zebrafish embryo with an antisense morpholino significantly altered pronephros development. Our results indicate that renal p75NTR is altered in CAKUT fetuses, and could participate to early nephrogenesis.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping