PUBLICATION
Sequence analysis and spatiotemporal developmental distribution of the Cat-1-type transporter slc7a1a in zebrafish (Danio rerio)
- Authors
- Ellingsen, S., Narawane, S., Fjose, A., Verri, T., Rønnestad, I.
- ID
- ZDB-PUB-201002-183
- Date
- 2020
- Source
- Fish physiology and biochemistry 46(6): 2281-2298 (Journal)
- Registered Authors
- Ellingsen, Ståle, Fjose, Anders
- Keywords
- Cationic amino acid transport(ers), Spatiotemporal expression, Zebrafish, slc7a1a, slc7a1b
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Brain/metabolism
- Branchial Region/metabolism
- Cationic Amino Acid Transporter 1/chemistry
- Cationic Amino Acid Transporter 1/genetics*
- Embryo, Nonmammalian
- Eye/metabolism
- Gene Expression Regulation, Developmental
- Nephrons/metabolism
- Phylogeny
- Sequence Analysis, DNA
- Sequence Analysis, Protein
- Somites/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics*
- PubMed
- 32980952 Full text @ Fish Physiol. Biochem.
Citation
Ellingsen, S., Narawane, S., Fjose, A., Verri, T., Rønnestad, I. (2020) Sequence analysis and spatiotemporal developmental distribution of the Cat-1-type transporter slc7a1a in zebrafish (Danio rerio). Fish physiology and biochemistry. 46(6):2281-2298.
Abstract
Cationic amino acid transporter 1 (Cat-1 alias Slc7a1) is a Na+-independent carrier system involved in transport and absorption of the cationic amino acids lysine, arginine, histidine, and ornithine and has also been shown to be indispensable in a large variety of biological processes. Starting from isolated full-length zebrafish (Danio rerio) cDNA for slc7a1a, we performed comparative and phylogenetic sequence analysis, investigated the conservation of the gene during vertebrate evolution, and defined tissue expression during zebrafish development. Whole mount in situ hybridization first detected slc7a1a transcripts in somites, eyes, and brain at 14 h post-fertilization (hpf) with additional expression in the distal nephron at 24 hpf and in branchial arches at 3 days post-fertilization (dpf), with significant increase by 5 dpf. Taken together, the expression analysis of the zebrafish Cat-1 system gene slc7a1a suggests a functional role(s) during the early development of the central nervous system, muscle, gills, and kidney. Graphical abstract.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping