PUBLICATION
5-Hydroxytryptamine (5-HT) Positively Regulates Pigmentation via Inducing Melanoblast Specification and Melanin Synthesis in Zebrafish Embryos
- Authors
- Liu, L., Zhong, M., Dong, J., Chen, M., Shang, J., Yue, Y.
- ID
- ZDB-PUB-201002-126
- Date
- 2020
- Source
- Biomolecules 10(9): (Journal)
- Registered Authors
- Keywords
- 5-hydroxytryptamine, melanocytes differentiation, melanocytes regeneration, protein kinase A signaling, zebrafish
- MeSH Terms
-
- Animals
- Cell Line, Tumor
- Dose-Response Relationship, Drug
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental/drug effects
- Larva/drug effects
- Larva/metabolism
- Larva/physiology
- Melanins/biosynthesis*
- Melanocytes/cytology
- Melanocytes/drug effects*
- Melanocytes/metabolism
- Microphthalmia-Associated Transcription Factor/genetics
- Microphthalmia-Associated Transcription Factor/metabolism
- Neural Crest/cytology
- Neural Crest/drug effects
- Neural Crest/metabolism
- Pigmentation/drug effects*
- Pigmentation/genetics
- Regeneration/drug effects
- Regeneration/genetics
- Serotonin/pharmacology*
- Stem Cells/cytology
- Stem Cells/drug effects*
- Stem Cells/metabolism
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 32961761 Full text @ Biomolecules
Citation
Liu, L., Zhong, M., Dong, J., Chen, M., Shang, J., Yue, Y. (2020) 5-Hydroxytryptamine (5-HT) Positively Regulates Pigmentation via Inducing Melanoblast Specification and Melanin Synthesis in Zebrafish Embryos. Biomolecules. 10(9):.
Abstract
It has been reported that 5-hydroxytryptamine (5-HT) is related to melanogenesis in mice and melanoma cells. However, the underlying mechanisms of 5-HT in regulating pigmentation remains unknown. In this study, we aim to clarify the regulatory mechanism of 5-HT in the pigmentation of zebrafish embryos and B16F10 cells. Our results show that 5-HT induces the pigmentation of zebrafish embryos in a dosage-dependent manner at concentrations of 0.01-1 mM. Whole mount in situ hybridizations and qRT-PCR in zebrafish embryos indicate that the expression of neural crest cells marker gene sox10 is not changed in embryos treated with 5-HT compared to control group. The expression of mitfa, the marker gene of melanoblasts, is increased in the presence of 5-HT. Furthermore, 5-HT increased the expression of regeneration associated genes, namely kita, mitfa, and dct, after ablation of the melanogenic cells in zebrafish embryos. The experiments in B16F10 cells show that 5-HT promotes melanin synthesis by up-regulating the expression of key proteins MITF, TYR, TRP-1, and TRP-2. Especially, the small molecule inhibitor of PKA signaling, but not AKT and MAPK signaling, attenuates the up-regulation of MITF and TYR resulted from 5-HT induction in B16F10 cells. These results will help us to further understand the regulatory network of vertebrate pigmentation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping