PUBLICATION
Naringenin alleviates 6-hydroxydopamine induced Parkinsonism in SHSY5Y cells and zebrafish model
- Authors
- Kesh, S., Kannan, R.R., Balakrishnan, A.
- ID
- ZDB-PUB-201002-100
- Date
- 2020
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 239: 108893 (Journal)
- Registered Authors
- Keywords
- Danio rerio, Mitochondrial dysfunction, Naringenin, Neuro-protection, Oxidative stress, Parkinson's disease
- MeSH Terms
-
- Animals
- Biomarkers/metabolism
- Cell Line
- Disease Models, Animal
- Flavanones/pharmacology*
- Humans
- Locomotion/drug effects*
- Membrane Potential, Mitochondrial/drug effects
- Neuroprotective Agents/pharmacology*
- Oxidative Stress/drug effects*
- Parkinsonian Disorders/drug therapy*
- Zebrafish
- PubMed
- 32949818 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Kesh, S., Kannan, R.R., Balakrishnan, A. (2020) Naringenin alleviates 6-hydroxydopamine induced Parkinsonism in SHSY5Y cells and zebrafish model. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 239:108893.
Abstract
6-Hydroxydopamine (6-OHDA) is a neurotoxin that inhibits the mitochondrial complex I causing mitochondrial impairment, aetiology of Parkinson's. Naringenin is a flavanone predominantly present in citrus fruits. Due to its high antioxidant and anti-inflammatory potential, it has been widely studied against various disorders. In this study, the neuroprotective effect of naringenin was determined against 6-OHDA induced toxicity with Levodopa (l-DOPA) as the standard. Naringenin reduced 6-OHDA induced oxidative stress biomarker levels such as CAT, GSH, SOD, and ROS. Naringenin rescued 6-OHDA induced reduction of the mitochondrial membrane potential. Treatment with naringenin improved the locomotion of the 6-OHDA treated zebrafish larvae which showed stagnant swimming patterns. Naringenin was also found to downregulate the expression of some Parkinsonian genes such as casp9, lrrk2, and polg and upregulate pink1. These studies attribute to naringenin as a viable molecule to study further for its neuroprotective effects against 6-OHDA induced neurotoxicity and neurodegeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping