PUBLICATION

Astrocytes influence medulloblastoma phenotypes and CD133 surface expression

Authors
Gronseth, E., Gupta, A., Koceja, C., Kumar, S., Kutty, R.G., Rarick, K., Wang, L., Ramchandran, R.
ID
ZDB-PUB-201002-1
Date
2020
Source
PLoS One   15: e0235852 (Journal)
Registered Authors
Ramchandran, Ramani
Keywords
none
MeSH Terms
  • AC133 Antigen/genetics*
  • AC133 Antigen/metabolism
  • Animals
  • Astrocytes/metabolism*
  • Cell Line, Tumor
  • Cell Movement
  • Tumor Microenvironment
  • Humans
  • Medulloblastoma/metabolism*
  • Cells, Cultured
  • Phenotype*
  • Zebrafish
  • Neoplastic Stem Cells/drug effects
  • Neoplastic Stem Cells/metabolism
  • Neoplastic Stem Cells/physiology
  • Cell Adhesion
  • Culture Media, Conditioned
  • Nestin/genetics
  • Nestin/metabolism
  • Octamer Transcription Factor-3/genetics
  • Octamer Transcription Factor-3/metabolism
(all 21)
PubMed
32628717 Full text @ PLoS One
Abstract
The medulloblastoma (MB) microenvironment is diverse, and cell-cell interactions within this milieu is of prime importance. Astrocytes, a major component of the microenvironment, have been shown to impact primary tumor cell phenotypes and metastasis. Based on proximity of MB cells and astrocytes in the brain microenvironment, we investigated whether astrocytes may influence MB cell phenotypes directly. Astrocyte conditioned media (ACM) increased Daoy MB cell invasion, adhesion, and in vivo cellular protrusion formation. ACM conditioning of MB cells also increased CD133 surface expression, a key cancer stem cell marker of MB. Additional neural stem cell markers, Nestin and Oct-4A, were also increased by ACM conditioning, as well as neurosphere formation. By knocking down CD133 using short interfering RNA (siRNA), we showed that ACM upregulated CD133 expression in MB plays an important role in invasion, adhesion and neurosphere formation. Collectively, our data suggests that astrocytes influence MB cell phenotypes by regulating CD133 expression, a key protein with defined roles in MB tumorgenicity and survival.
Genes / Markers
Figures
Figure Gallery (4 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b140
    		Unknown
    b692
      		Point Mutation
      y171TgTransgenic Insertion
        1 - 3 of 3
        Show
        Human Disease / Model
        Human Disease Fish Conditions Evidence
        medulloblastomaTAS
        1 - 1 of 1
        Show
        Sequence Targeting Reagents
        No data available
        Fish
        Fish
        mitfab692/b692; ednrbab140/b140; y171Tg
        1 - 1 of 1
        Show
        Antibodies
        No data available
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        mCherryEFGmCherry
        1 - 1 of 1
        Show
        Mapping
        No data available
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        Citation
        Gronseth, E., Gupta, A., Koceja, C., Kumar, S., Kutty, R.G., Rarick, K., Wang, L., Ramchandran, R. (2020) Astrocytes influence medulloblastoma phenotypes and CD133 surface expression. PLoS One. 15:e0235852.