PUBLICATION
Developmental neurotoxicity fingerprint of silica nanoparticles at environmentally relevant level on larval zebrafish using a neurobehavioral-phenomics-based biological warning method
- Authors
- Li, X., Dang, J., Li, Y., Wang, L., Li, N., Liu, K., Jin, M.
- ID
- ZDB-PUB-200906-15
- Date
- 2020
- Source
- The Science of the total environment 752: 141878 (Journal)
- Registered Authors
- Keywords
- Biological warning method, Environmentally relevant concentration, Fish embryos toxicity (FET), Light/dark challenge (LDC), Locomotion response (LMR), Neurobehavioral phenomics (NBP)
- MeSH Terms
-
- Zebrafish
- Phenomics
- Larva
- Silicon Dioxide/toxicity
- Nanoparticles*
- Behavior, Animal
- Embryo, Nonmammalian
- Animals
- Water Pollutants, Chemical*/toxicity
- PubMed
- 32890834 Full text @ Sci. Total Environ.
Citation
Li, X., Dang, J., Li, Y., Wang, L., Li, N., Liu, K., Jin, M. (2020) Developmental neurotoxicity fingerprint of silica nanoparticles at environmentally relevant level on larval zebrafish using a neurobehavioral-phenomics-based biological warning method. The Science of the total environment. 752:141878.
Abstract
Background Larval zebrafish (Danio rerio) is not only an ideal vertebrate applied in Fish Embryos Toxicity (FET) test but also a well-accepted model in behavioral neurotoxicity research. By applying the commercial standard behavioral tracking system (Zebrabox), the locomotion profiles (neurobehavioral-phenomics) of larval zebrafish can be comprehensively monitored and systematically analyzed to probe ecotoxicological neurotoxicity of nano-pollutants at environmental relevant concentration level.
Results Herein, the potential toxicity of at environment relevant concentration level on embryonic zebrafish was evaluated by FET and neurobehavioral-phenomics (NBP). The embryos were exposed to the environmental relevant concentration (0.05, 0.1,1, 5, 10, 100 μg/L). The FET criteria were utilized to evaluate the ecotoxicological effect induced by silica NPs. Subsequently, behavioral neurotoxicity of silica NPs was further quantified via locomotion response (LMR). Specifically, the alteration of Light/Dark challenge (LDC) evoked by light/dark stimulation was detected and analyzed by commercially standard behavioral protocols using zebrabox. We revealed that the exposures of silica NPs at environmental relevant concentration (0.05, 0.1, 1, 5, 10,100 μg/L) significantly disturbed locomotion profiles of larval zebrafish. Additionally, it was obviously noted that low, environmentally relevant silica concentrations might result in altering the total behavioral profiles in developing zebrafish.
Conclusions In sum, neurobehavior phenomics profiling based on LMR and LDC is a potent methodology for the evaluation of sub-lethal or sub-teratogenic toxicity. Compared with the FET tests characterized by the detection of embryonic teratogenicity, the neurobehavior phenomics based method can be more sensitive to determine sub-teratogenic toxicity of silica NPs at environmental concentrations. With the combination of multivariate data analysis, this approach would offer effective technical reference for environmental nano-toxicology research.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping