PUBLICATION

The Dynamics of Epidermal Stratification During Post-larval Development in Zebrafish

Authors
Rangel-Huerta, E., Guzman, A., Maldonado, E.
ID
ZDB-PUB-200904-10
Date
2020
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   250(2): 175-190 (Journal)
Registered Authors
Maldonado, Ernesto
Keywords
Asymmetric cell division, Epidermal stratification, Progenitors, Stem cells, Zebrafish
MeSH Terms
  • Phosphoproteins/metabolism
  • Cell Differentiation
  • Zebrafish/growth & development*
  • Zebrafish/metabolism
  • Epidermis/growth & development*
  • Epidermis/metabolism
  • Trans-Activators/metabolism
  • Epidermal Cells/cytology
  • Zebrafish Proteins/metabolism
  • Animals
(all 10)
PubMed
32877571 Full text @ Dev. Dyn.
Abstract
Background The epidermis, as a defensive barrier, is a consistent trait throughout animal evolution. During post-larval development, the zebrafish epidermis thickens by stratification or addition of new cell layers. Epidermal basal stem cells, expressing the transcription factor p63, are known to be involved in this process. Zebrafish post-larval epidermal stratification is a tractable system to study how stem cells participate in organ growth.
Methods We used immunohistochemistry, in combination with EdU cell proliferation detection, to study zebrafish epidermal stratification. For this procedure, we selected a window of post-larval stages (5 - 8 mm of standard length or SL, which normalizes age by size). Simultaneously, we used markers for asymmetric cell division and the Notch signaling pathway.
Results We found that epidermal stratification is the consequence of two events, changes in cell shape and active cell proliferation. We identified a subset of highly proliferative epidermal cells with reduced levels of p63, which differed from the basal stem cells with high levels of p63. Additionally, we described different mechanisms that participate in the stratification process, including the phosphorylation of p63, asymmetric cell division regulated by the Par3 and LGN proteins, and expression of Notch genes. This article is protected by copyright. All rights reserved.
Genes / Markers
Figures
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a9
    		Complex
    w2
      		Point Mutation
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      No data available
      Antibodies
      Name Type Antigen Genes Isotypes Host Organism
      Ab1-gpsm2polyclonal
        		IgGRabbit
        Ab2-krtmonoclonal
          		IgG1Mouse
          Ab2-pard3polyclonal
            		IgGRabbit
            Ab46-h3polyclonal
              		IgGRabbit
              1 - 4 of 4
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              Orthology
              No data available
              Engineered Foreign Genes
              No data available
              Mapping
              No data available
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              Citation
              Rangel-Huerta, E., Guzman, A., Maldonado, E. (2020) The Dynamics of Epidermal Stratification During Post-larval Development in Zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 250(2):175-190.