PUBLICATION
Lipid peroxidation regulates long-range wound detection through 5-lipoxygenase in zebrafish
- Authors
- Katikaneni, A., Jelcic, M., Gerlach, G.F., Ma, Y., Overholtzer, M., Niethammer, P.
- ID
- ZDB-PUB-200902-6
- Date
- 2020
- Source
- Nature cell biology 22(9): 1049-1055 (Journal)
- Registered Authors
- Niethammer, Philipp
- Keywords
- none
- MeSH Terms
-
- Animals
- Arachidonate 5-Lipoxygenase/metabolism*
- Arachidonic Acid/metabolism
- Leukocytes/metabolism
- Lipid Peroxidation/physiology*
- Oxidation-Reduction
- Reactive Oxygen Species/metabolism
- Wounds and Injuries/metabolism*
- Zebrafish/metabolism*
- PubMed
- 32868902 Full text @ Nat. Cell Biol.
Citation
Katikaneni, A., Jelcic, M., Gerlach, G.F., Ma, Y., Overholtzer, M., Niethammer, P. (2020) Lipid peroxidation regulates long-range wound detection through 5-lipoxygenase in zebrafish. Nature cell biology. 22(9):1049-1055.
Abstract
Rapid wound detection by distant leukocytes is essential for antimicrobial defence and post-infection survival1. The reactive oxygen species hydrogen peroxide and the polyunsaturated fatty acid arachidonic acid are among the earliest known mediators of this process2-4. It is unknown whether or how these highly conserved cues collaborate to achieve wound detection over distances of several hundreds of micrometres within a few minutes. To investigate this, we locally applied arachidonic acid and skin-permeable peroxide by micropipette perfusion to unwounded zebrafish tail fins. As in wounds, arachidonic acid rapidly attracted leukocytes through dual oxidase (Duox) and 5-lipoxygenase (Alox5a). Peroxide promoted chemotaxis to arachidonic acid without being chemotactic on its own. Intravital biosensor imaging showed that wound peroxide and arachidonic acid converged on half-millimetre-long lipid peroxidation gradients that promoted leukocyte attraction. Our data suggest that lipid peroxidation functions as a spatial redox relay that enables long-range detection of early wound cues by immune cells, outlining a beneficial role for this otherwise toxic process.
Genes / Markers
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
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