PUBLICATION

Tracking Neutrophil Migration in Zebrafish Model Using Multi-Channel Feature Learning

Authors
Rahmani Moghadam, M., Chen, Y.P.
ID
ZDB-PUB-200828-31
Date
2020
Source
IEEE journal of biomedical and health informatics   25(4): 1197-1205 (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • Animals
  • Neural Networks, Computer
  • Neutrophils*
  • Zebrafish*
PubMed
32853155 Full text @ IEEE J Biomed Health Inform
Abstract
Tracking cells over time is crucial in the fields of computer vision and biomedical science. Studying neutrophils and their migratory profile is the highly topical fields in inflammation research due to determining role of these cells during immune responses. As neutrophils generally are of various shapes and motion, it remains challenging to track and describe their behaviours from multi-dimensional microscopy datasets. In this study, we propose a robust novel multi-channel feature learning (MCFL) model inspired by deep learning to extract the complex behaviour of neutrophils moved in time lapse images. In this model, the convolutional neural networks along with cell relocation distance and orientation channels learn the robust significant spatial and temporal features of an individual neutrophil. Additionally, we also proposed a new cell tracking framework to detect and track neutrophils in the original time-laps microscopy images, entails sampling, observation, and visualisation functions. Our proposed cell tracking-based-multi channel feature learning method has remarkable performance in rectifying common cell tracking problem compared with state-of the-art methods.
Genes / Markers
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping